Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection 英文参考文献.docVIP

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Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection 英文参考文献.doc

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Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection 英文参考文献

GenerationandCharacterizationoftheWestern RegionalResearchCenterBrachypodiumT-DNA InsertionalMutantCollection JenniferN.Bragg1,2.,JiajieWu1,2.¤,SeanP.Gordon1,MaraE.Guttman1,RogerThilmony1, GerardR.Lazo1,YongQ.Gu1,JohnP.Vogel1* 1United States Department of Agriculture- Agriculture Research Service (USDA-ARS), Western Regional Research Center, Albany, California, United States of America, 2UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica Abstract ThemodelgrassBrachypodiumdistachyon(Brachypodium)isanexcellentsystemforstudyingthebasicbiologyunderlying traitsrelevanttotheuseofgrassesasfood,forageandenergycrops.ToaddtothegrowingcollectionofBrachypodium resources available to plant scientists, we further optimized our Agrobacterium tumefaciens-mediated high-efficiency transformationmethodandgenerated8,491BrachypodiumT-DNAlines.WeusedinversePCRtosequencetheDNAflanking theinsertionsitesinthemutants.Usingtheseflankingsequencetags(FSTs)wewereabletoassign7,389FSTsfrom4,402T- DNA mutants to 5,285 specific insertion sites (ISs) in the Brachypodium genome. More than 29% of the assigned ISs are supported by multiple FSTs. T-DNA insertions span the entire genome with an average of 19.3 insertions/Mb. The distribution of T-DNA insertions is non-uniform with a larger number of insertions at the distal ends compared to the centromericregionsofthechromosomes.Insertionsarecorrelatedwithgenicregions,butarebiasedtowardUTRsandnon- codingregionswithin1kbofgenesoverexonsandintronregions.Morethan1,300uniquegeneshavebeentaggedinthis population. Information about the Western Regional Research Center Brachypodium insertional mutant population is available on a searchable website () designed to provide researchers with a means to orderT-DNAlineswithmutationsingenesofinterest. Citation: Bragg JN, Wu J, Gordon SP, Guttman ME, Thilmony R, et al. (2012) Generation and Characterization of the Western Regional Research Center BrachypodiumT-DNAInsertionalMutantCollection.PLoSONE7(9):e