棉花GhFTL1基因的克隆及初步功能分析.pdfVIP

Cotton Scíence 棉花学报 Cotωn Science 2011 ,23(6):515-521 棉花 GhFTLl 基因的克隆及初步功能分析 东锐，院海英，顾超，郑银英，黄先忠\崔百明· (石河子大学生命科学学院农业生物技术重点实验室，新疆石河子 832003) 摘要:通过 RT-PCR 结合 RACE 技术，从新疆陆地棉品种新陆早 33 中克隆到一个 FT 类似基因，命名为 GhFTLI 基因(登录号 :HM631972)。该基因 ORF(Open Reading Frame)全长为 525 bp ，可编码 174 个氨基酸，与 其它植物中克隆的 FT 同源蛋白高度相似，含有 FT 蛋白亚家族两个关键的氨基酸残基及保守氨基酸基序。该 基因在根、茎、花、叶片、纤维和胚珠中均有表达，且在叶片和胚珠中的表达要稍高于其它组织。系统进化分析 表明 GhFTLl 属于 FT 亚家族成员。在 18 个已经证明可以促进植物开花的 FT 同源基因中 ， GhF丁Ll同苹果的 MdFTl的遗传距离最接近。在拟南芥中过量表达 GhFTLI ， T) 转基因植株要比野生型明显提早开花。表明 GhFTLl 可能属于开花途径中的重要作用因子之一。 关键词: GhFTLl ;Flowering Locus T; 成花素;促早开花;陆地棉 中图分类号:S562.035 .3 文献标志码:A 文章编号: 1002-7807(2011 )06-0515-07 Cloning and Primary Analysis of the Function of GhFTL1 Gene in Cotton (Gossypium hirsutum L.) DONG Rui , YUAN Hai-ying, GU Chao , ZHENG Yin-ying, HUANG Xian-zhong. , CUI Bai-ming. (Key Laboratory ofAgrobio阳hnology， College ofLife Science, Shihezi Universi秽 Shih钮， Xinjiang 832003 , China) Abstract: A FT- Iike homolog was isolated from Gossypium hirsu阳m L. CV. X in1uzao 33 伽rough RT-PCR and RACE , which was named GhFTL I gene (aιcession number: HM631972). The 525-bp open reading frame encoded a putative protein of 174 amino acids wi伽 high similarity to FT proteins of different plants which contains two critical residues and conserved motif, bo也 of which identified as diagnostic of true FT. GhFTL I gene showed constitutive expression in root, stem, flower, leaf, fiber, and ovule. The levels of transcript in leaf and ovule were higher 也an that in other tissues. Phylogenetic analysis at the amino acid level confirmed 阳t the isolated sequence is FT-l汰e， and also showed that GhFTLl had shorter genetic distance with MdFTl compared with other 18 FT homologs confirmed to inducing early flowering 企om 13 different plants. Over-expression of GhFTLl in Arabidopsis u