Global Expression of Cell Surface Proteins in Embryonic Stem Cells 英文参考文献.docVIP

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Global Expression of Cell Surface Proteins in Embryonic Stem Cells 英文参考文献.doc

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Global Expression of Cell Surface Proteins in Embryonic Stem Cells 英文参考文献

GlobalExpressionofCellSurfaceProteinsinEmbryonic StemCells BinGu1,JiarongZhang1,WeiWang1,LijuanMo1,YangZhou1,LiangbiaoChen2,YusenLiu3 ,Ming Zhang1* 1The Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Hangzhou, China, 2The Institute of Genetics and Developmental Biology, ChineseAcademicofSciences,Beijing,China,3CenterforPerinatalResearch,TheResearchInstituteatNationwideChildren’sHospital,DepartmentofPediatrics,TheOhio StateUniversityCollegeofMedicine,Columbus,Ohio,UnitedStatesofAmerica Abstract Background:Recentstudieshaveshownthatembryonicstem(ES)cellsgloballyexpressmostgenesinthegenomeatthe mRNAlevel;however,itisunclearwhetherthisglobalexpressionispropagatedtotheproteinlevel.Cellsurfaceproteins couldperformcriticalfunctionsinEScells,sodeterminingwhetherEScellsgloballyexpresscellsurfaceproteinswouldhave significantimplicationsforEScellbiology. MethodsandPrincipalFindings:ThesurfaceproteinsofmouseEScellswerepurifiedbybiotinlabelingandsubjectedto proteomicsanalysis.About1000transmembraneorsecretedcellsurfaceproteinswereidentified.Theseproteinscovereda large variety if functional categories including signal transduction, adhesion and transporting. More over, mES cells promiscuously expressed a wide variety of tissue specific surface proteins. And many surface proteins were expressed heterogeneouslyonmEScells.WealsofindthathumanEScellsexpressawidevarietyoftissuespecificsurfaceproteins. Conclusions/Significance:Ourresultsindicatethatglobalgeneexpressionisnotsimplyaresultofleakygeneexpression, whichcouldbeattributedtotheloosechromatinstructureofEScells;itisalsopropagatedtothefunctionallevel.EScells may use diverse surface proteins to receive signals from the diverse extracellular stimuli that initiate differentiation. Moreover,thepromiscuousexpressionoftissuespecificsurfaceproteinsilluminatenewinsightsintothestrategiesofcell surfacemarkerscreening. Citation: Gu B, Zhang J, Wang W, Mo L, Zhou Y, et al. (2010) Global Expression of C