海南芒果露水斑病的初步鉴定.pdfVIP

75 广东农业科学 2013 年第 7 期 海南芒果露水斑病的初步鉴定 张贺1 ，刘晓妹2 ，喻群芳1 ，漆艳香1 ，谢艺贤1 ，蒲金基1 ，2 (1.中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室，海南海口 571101 ; 2.海南大学环境与植物保护学院，海南海口 570228) 摘要:芒果露水斑病为海南省新发现的病害，该病主要为害果实，多在采收期显症，病斑呈不规则形的水溃状;湿度大时，病 斑上产生墨绿色的霉层，严重影响果实的外观和商品价值。通过该病害病原菌柯赫氏法则验证、形态学鉴定和病原菌的rDNA-I凹 的序列比对分析，确定病原菌为Cladosporium cladosporioides (枝状芽枝霉)，其rDNA-I15序列长为511 bp ,GenBank 登录号 HM8566220 芒果露水斑病在海南省属首次报道。 关键词:芒果露水斑病; Cladosporium c[，缸losporioides ; 鉴定 文章编号:1ω4-874X(2013 )07-∞75-03 中图分类号:5682.2 文献标识码:A Preliminary studies of mango sooty blotch of Cladosporium cladosporioides in Hainan province l 2 l 1 1 1 ZHANG He , UU Xiao-mei , YU Qun-fang , QI Yan-xiang , XIE Yi-xian , PU Jin-ji ,2 (1. Environment arui Plant 丹otection Instit脚， CATAS lKey Lahor脚ry o/IntegraJed Pest Management on Tropical Crops, Ministry 0/ Agriculture , Haikou 571101 , Chùw; 2. College 0/ Environment αnd Plant 丹otection， Hainan University, Haikou 570228, China) Abstract: A new disease that mainly infected fruits in harvesting time locally called mango s∞ty blotch was found in Hainan province of China The symptom appeared 皿 a irregular dark, clouded stain on pee l.There were green mold layer on the spots under the conditions of high humidity, which seriously affect the appearance of the fruit and caused great lose of commodity value. The fungus w副 identified to be Cladosporium cladosporioides (Fr.) de Vries according to its morphology and the sequence analysis of rDNA-I 15. And its sequence of rDNA- 15 was 511 b