Large Scale Library Generation for High Throughput Sequencing 英文参考文献.docVIP

LargeScaleLibraryGenerationforHighThroughput Sequencing ErikBorgstro¨m., SverkerLundin.,JoakimLundeberg* ScienceforLifeLaboratory,RoyalInstituteofTechnology(KTH),SchoolofBiotechnology,DivisionofGeneTechnology,Solna,Sweden Abstract Background:Largeeffortshaverecentlybeenmadetoautomatethesamplepreparationprotocolsformassivelyparallel sequencing in order to match the increasing instrument throughput. Still, the size selection through agarose gel electrophoresisseparationisalabor-intensivebottleneckoftheseprotocols. Methodology/PrincipalFindings:Inthisstudyamethodforautomaticlibrarypreparationa