Metagenomic Profiling of Microbial Composition and Antibiotic Resistance Determinants in Puget Sound 英文参考文献.docVIP

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Metagenomic Profiling of Microbial Composition and Antibiotic Resistance Determinants in Puget Sound 英文参考文献.doc

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Metagenomic Profiling of Microbial Composition and Antibiotic Resistance Determinants in Puget Sound 英文参考文献

MetagenomicProfilingofMicrobialCompositionand AntibioticResistanceDeterminantsinPugetSound JesseA.Port1,2,JamesC.Wallace1,2,WilliamC.Griffith1,2,ElaineM.Faustman1,2 * 1Department of Environmental and Occupational Health Sciences, School of Public Health, University ofWashington, Seattle, Washington,United States of America, 2InstituteforRiskAnalysisandRiskCommunication,SchoolofPublicHealth,UniversityofWashington,Seattle,Washington,UnitedStatesofAmerica Abstract Human-health relevant impacts on marine ecosystems are increasing on both spatial and temporal scales. Traditional indicators for environmental health monitoring and microbial risk assessment have relied primarily on single species analysesandhaveprovidedonlylimitedspatialandtemporalinformation.Morehigh-throughput,broad-scaleapproaches toevaluatetheseimpactsarethereforeneededtoprovideaplatformforinformingpublichealth.Thisstudyusesshotgun metagenomics to survey the taxonomic composition and antibiotic resistance determinant content of surface water bacterialcommunitiesinthePugetSoundestuary.MetagenomicDNAwascollectedatsixsitesinPugetSoundinaddition toonewastewatertreatmentplant(WWTP)thatdischargesintotheSoundandpyrosequenced.Atotalof,550Mbp(1.4 million reads) were obtained, 22Mbp of which could be assembled into contigs. While the taxonomic and resistance determinantprofilesacrosstheopenSoundsamplesweresimilar,uniquesignatureswereidentifiedwhencomparingthese profiles across the open Sound, a nearshore marina and WWTP effluent. The open Sound was dominated by a- Proteobacteria (in particular Rhodobacterales sp.), c-Proteobacteria and Bacteroidetes while the marina and effluent had increasedabundancesofActinobacteria,b-ProteobacteriaandFirmicutes.Therewasasignificantincreaseintheantibiotic resistancegenesignalfromtheopenSoundtomarinatoWWTPeffluent,suggestiveofapotentiallinktohumanimpacts. Mobilegeneticelementsassociatedwithenvironmentalandpathogenicbacteriawerealsodifferentiallyabundantacross thesamples.Thisst