Metal A and Metal B Sites of Nuclear RNA Polymerases Pol IV and Pol V Are Required for siRNA-Dependent DNA Methylation and Gene Silencing 英文参考文献.docVIP

下载本文档

6
0
约4.63万字
约 10页
2017-05-12 发布于上海
举报
版权申诉

Metal A and Metal B Sites of Nuclear RNA Polymerases Pol IV and Pol V Are Required for siRNA-Dependent DNA Methylation and Gene Silencing 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Metal A and Metal B Sites of Nuclear RNA Polymerases Pol IV and Pol V Are Required for siRNA-Dependent DNA Methylation and Gene Silencing 英文参考文献

MetalAandMetalBSitesofNuclearRNAPolymerases PolIVandPolVAreRequiredforsiRNA-DependentDNA MethylationandGeneSilencing JeremyR.Haag,OlgaPontes,CraigS.Pikaard* DepartmentofBiology,WashingtonUniversity,St.Louis,Missouri,UnitedStatesofAmerica Abstract Plants are unique among eukaryotes in having five multi-subunit nuclear RNA polymerases: the ubiquitous RNA polymerases I, II and III plus two plant-specific activities, nuclear RNA polymerases IV and V (previously known as PolymerasesIVaandIVb).PolIVandPolVarenotrequiredforviabilitybutplaynon-redundantrolesinsmallinterfering RNA(siRNA)-mediatedpathways,includingapathwaythatsilencesretrotransposonsandendogenousrepeatsviasiRNA- directedDNAmethylation.RNApolymeraseactivityhasnotbeendemonstratedforPolymerasesIVorVinvitro,makingit unclearwhethertheyarecatalyticallyactiveenzymes.Theirlargestandsecond-largestsubunitsequenceshavediverged considerablyfromPolI,IIandIIIinthevicinityofthecatalyticcenter,yetretaintheinvariantMetalAandMetalBaminoacid motifsthatbindmagnesiumionsessentialforRNApolymerization.Byusingsite-directedmutagenesisinconjunctionwith in vivo functional assays, we show that the Metal A and Metal B motifs of Polymerases IV and V are essential for siRNA production, siRNA-directed DNA methylation, retrotransposon silencing, and the punctate nuclear localization patterns typicalofbothpolymerases.Collectively,thesedatashowthattheminimalcoresequencesofpolymeraseactivesites,the MetalAandBsites,areessentialforPolIVandPolVbiologicalfunctions,implyingthatbotharecatalyticallyactive. Citation:HaagJR,PontesO,PikaardCS(2009)MetalAandMetalBSitesofNuclearRNAPolymerasesPolIVandPolVAreRequiredforsiRNA-DependentDNA MethylationandGeneSilencing.PLoSONE4(1):e4110.doi:10.1371/journal.pone.0004110 Editor:FredericBerger,TemasekLifeSciencesLaboratory,Singapore ReceivedOctober8,2008;AcceptedNovember30,2008;PublishedJanuary1,2009 Copyright: ? 2009 Haag et al. This is an open-access article distributed under the terms of the Creative Commons A