Mice Deficient in Ribosomal Protein S6 Phosphorylation Suffer from Muscle Weakness that Reflects a Growth Defect and Energy Deficit 英文参考文献.docVIP

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Mice Deficient in Ribosomal Protein S6 Phosphorylation Suffer from Muscle Weakness that Reflects a Growth Defect and Energy Deficit 英文参考文献.doc

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Mice Deficient in Ribosomal Protein S6 Phosphorylation Suffer from Muscle Weakness that Reflects a Growth Defect and Energy Deficit 英文参考文献

MiceDeficientinRibosomalProteinS6Phosphorylation SufferfromMuscleWeaknessthatReflectsaGrowth DefectandEnergyDeficit IgorRuvinsky1.¤a,MaximilianoKatz1.¤b,AvigailDreazen1,YuvalGielchinsky2,AnnSaada3 ,Nanette Freedman4,EyalMishani4,GabrielZimmerman5,JudithKasir1,OdedMeyuhas1* 1DepartmentofBiochemistryandMolecularBiology,IMRIC,HebrewUniversity-HadassahMedicalSchool,Jerusalem,Israel,2DepartmentofObstetricsandGynecology, Hadassah Medical Center, Jerusalem, Israel, 3Metabolic Disease Unit, Hadassah Medical Center, Jerusalem, Israel, 4Department of Medical Biophysics and Nuclear Medicine,HadassahMedicalCenter,Jerusalem,Israel,5DepartmentofBiologicalChemistry,InstituteofLifeSciences,TheHebrewUniversity,Jerusalem,Israel Abstract Background:Mice,whoseribosomalproteinS6cannotbephosphorylatedduetoreplacementofallfivephosphorylatable serine residues by alanines (rpS6P2/2), are viable and fertile. However, phenotypic characterization of these mice and embryofibroblastsderivedfromthem,hasestablishedtheroleofthesemodificationsintheregulationofthesizeofseveral celltypes,aswellaspancreatic b-cellfunctionandglucosehomeostasis. Arelativelypassivebehaviorofthesemicehas raisedthepossibility thattheysufferfrommuscleweakness,whichhas,indeed,beenconfirmedbyavarietyofphysical performancetests. Methodology/PrincipalFindings:Alargevarietyofexperimentalmethodologies,includingmorphometricmeasurements of histological preparations, high throughput proteomic analysis, positron emission tomography (PET) and numerous biochemical assays, were used in an attempt to establish the mechanism underlying the relative weakness of rpS6P2/2 muscles.Collectively,theseexperimentshavedemonstratedthatthephysicalinferiorityappearstoresultfromtwodefects: a)adecreaseintotalmusclemassthatreflectsimpairedgrowth,ratherthanaberrantdifferentiationofmyofibers,aswellas a diminished abundance of contractile proteins; and b) a reduced content of ATP and phosphocreatine, two readily available energy sources. The abundanc