Methanocella conradii sp. nov., a Thermophilic, Obligate Hydrogenotrophic Methanogen, Isolated from Chinese Rice Field Soil 英文参考文献.docVIP

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Methanocella conradii sp. nov., a Thermophilic, Obligate Hydrogenotrophic Methanogen, Isolated from Chinese Rice Field Soil 英文参考文献.doc

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Methanocella conradii sp. nov., a Thermophilic, Obligate Hydrogenotrophic Methanogen, Isolated from Chinese Rice Field Soil 英文参考文献

Methanocellaconradiisp.nov.,aThermophilic,Obligate HydrogenotrophicMethanogen,IsolatedfromChinese RiceFieldSoil ZheLu¨,YahaiLu* CollegeofResourcesandEnvironmentalSciences,ChinaAgriculturalUniversity,Beijing,China Abstract Background:Methanocellalescontributessignificantlytoanthropogenicmethaneemissionsthatcauseglobalwarming,but fewpureculturesforMethanocellalesareavailabletopermitsubsequentlaboratorystudies(physiology,biochemistry,etc.). Methodology/Principal Findings: By combining anaerobic culture and molecular techniques, a novel thermophilic methanogen, strain HZ254T was isolated from a Chinese rice field soil located in Hangzhou, China. The phylogenetic analysesofboththe16SrRNAgeneandmcrAgene(encodingtheasubunitofmethyl-coenzymeMreductase)confirmed its affiliation with Methanocellales, and Methanocella paludicola SANAET was the most closely related species. Cells were non-motilerods,albeitwithaflagellum,1.4–2.8 mmlongandby0.2–0.3mminwidth.Theygrewat37–60uC(optimallyat 55uC)andsalinityof0–5gNaCll (optimallyat0–1gNaCll ).ThepHrangeforgrowthwas6.4–7.2(optimum6.8).Under theoptimumgrowthcondition,thedoublingtimewas6.5–7.8h,whichistheshortesteverobservedinMethanocellales. 21 21 StrainHZ254TutilizedH2/CO2butnotformateforgrowthandmethaneproduction.TheDNAG Ccontentofthisorganism + T T was52.7mol%.Thesequenceidentitiesof16SrRNAgeneandmcrAgenebetweenstrainHZ254 andSANAE were95.0 and87.5%respectively, andthegenomebased AverageNucleotideIdentityvaluebetweenthemwas74.8%.Thesetwo strainsdifferedinphenotypicfeatureswithregardtosubstrateutilization,possessionofaflagellum,doublingtime(under optimalconditions),NaClandtemperatureranges.Takingaccountofthephenotypicandphylogeneticcharacteristics,we propose strain HZ254T as a representative of a novel species, Methanocella conradii sp. nov. The type strain is HZ254T (=CGMCC1.5162T=JCM17849T=DSM24694T). Conclusions/Significance: Strain HZ254T could potentially serve as an excellent laboratory model for s