Metagenome Plasticity of the Bovine Abomasal Microbiota in Immune Animals in Response to Ostertagia Ostertagi Infection 英文参考文献.docVIP

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Metagenome Plasticity of the Bovine Abomasal Microbiota in Immune Animals in Response to Ostertagia Ostertagi Infection 英文参考文献.doc

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Metagenome Plasticity of the Bovine Abomasal Microbiota in Immune Animals in Response to Ostertagia Ostertagi Infection 英文参考文献

MetagenomePlasticityoftheBovineAbomasal MicrobiotainImmuneAnimalsinResponsetoOstertagia OstertagiInfection RobertW.Li1*,SitaoWu1,WeizhongLi2,YingHuang2,LouisC.Gasbarre1 1AnimalandNaturalResourcesInstitute,UnitedStatesDepartmentofAgriculture,AgriculturalResearchService,Beltsville,Maryland,UnitedStatesofAmerica,2Center forResearchinBiologicalSystems,UniversityofCaliforniaSanDiego,SanDiego,LaJolla,California,UnitedStatesofAmerica Abstract InfectionsincattlebytheabomasalnematodeOstertagiaostertagiresultinimpairedgastrointestinalfunction.Sixpartially immuneanimalsweredevelopedusingmultipledrug-attenuatedinfections, andthese animalsdisplayedreducedworm burdensandaslightlyelevatedabomasalpHuponreinfection.Inthisstudy,wecharacterizedtheabomasalmicrobiotain responsetoreinfectionusingmetagenomictools.Comparedtouninfectedcontrols,infectiondidnotinduceasignificant changeinthemicrobialcommunitycompositioninimmuneanimals.16SrRNAgene-basedphylogeneticanalysisidentified 15 phyla in the bovine abomasal microbiota with Bacteroidetes (60.5%), Firmicutes (27.1%), Proteobacteria (7.2%), Spirochates (2.9%), and Fibrobacteres (1.5%) being the most predominant. The number of prokaryotic genera and operational taxonomic units (OTU) identified in the abomasal microbial community was 70.8619.8 (mean 6 SD) and 90.362.9, respectively. However, the core microbiome comprised of 32 genera and 72 OTU. Infection seemingly had a minimalimpact on theabomasalmicrobial diversity at agenuslevel inimmune animals.Proteinspredicted fromwhole genome shotgun (WGS) DNA sequences were assigned to 5,408 Pfam and 3,381 COG families, demonstrating dazzling arrays of functional diversity in bovine abomasal microbial communities. However, none of COG functional classes were significantlyimpactedbyinfection.Ourresultsdemonstratethatimmuneanimalsmaydevelopabilitiestomaintainproper stabilityoftheirabomasalmicrobialecosystem.Aminimaldisruptioninthebovineabomasalmicrobiotabyreinfectionmay contributeequallytotheres