MicroRNA Expression and Identification of Putative miRNA Targets in Ovarian Cancer 英文参考文献.docVIP

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MicroRNA Expression and Identification of Putative miRNA Targets in Ovarian Cancer 英文参考文献.doc

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MicroRNA Expression and Identification of Putative miRNA Targets in Ovarian Cancer 英文参考文献

MicroRNAExpressionandIdentificationofPutative miRNATargetsinOvarianCancer NeetuDahiya1,CherylA.Sherman-Baust1,Tian-LiWang2,BenDavidson3,Ie-MingShih2 ,Yongqing Zhang4,WilliamWoodIII4,KevinG.Becker4,PatriceJ.Morin1,2* 1LaboratoryofCellularandMolecularBiology,HopkinsMedicalInstitutions,Baltimore,Maryland,UnitedStatesofAmerica,2DepartmentofPathology,JohnsHopkins MedicalInstitutions,Baltimore,Maryland,UnitedStatesofAmerica,3NorwegianRadiumHospital,Oslo,Norway,4ResearchResourceBranch,NationalInstituteonAging, Baltimore,Maryland,UnitedStatesofAmerica Abstract Background:MicroRNAs(miRNAs)representaclassofsmallnon-codingRNAsthatcontrolgeneexpressionbytargeting mRNAs and triggering either translation repression or RNA degradation. Emerging evidence suggests the potential involvementofaltered regulationofmiRNAinthepathogenesisofcancers,andthesegenesarethoughttofunctionas bothtumorsuppressorsandoncogenes. Methodology/PrincipalFindings:UsingmicroRNAmicroarrays,weidentifyseveralmiRNAsaberrantlyexpressedinhuman ovariancancertissuesandcelllines.miR-221standsoutasahighlyelevatedmiRNAinovariancancer,whilemiR-21and severalmembersofthelet-7familyarefounddownregulated.Publicdatabaseswereusedtorevealpotentialtargetsforthe highlydifferentiallyexpressedmiRNAs.Inordertoexperimentallyidentifytranscriptswhosestabilitymaybeaffectedbythe differentiallyexpressedmiRNAs,wetransfectedprecursormiRNAsintohumancancercelllinesandusedoligonucleotide microarraystoexaminechangesinthemRNAlevels.Interestingly,therewaslittleoverlapbetweenthepredictedandthe experimental targets or pathways, or between experimental targets/pathways obtained using different cell lines, highlightingthecomplexityofmiRNAtargetselection. Conclusion/Significance: Our results identify several differentially expressed miRNAs in ovarian cancer and identify potentialtargettranscriptsthatmayberegulatedbythesemiRNAs.ThesemiRNAsandtheirtargetsmayhaveimportant rolesintheinitiationanddevelopmentofovariancancer. Citation:DahiyaN,Sherman-B