Molecular Approach to the Identification of Fish in the South China Sea 英文参考文献.docVIP

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Molecular Approach to the Identification of Fish in the South China Sea 英文参考文献.doc

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Molecular Approach to the Identification of Fish in the South China Sea 英文参考文献

MolecularApproachtotheIdentificationofFishinthe SouthChinaSea JunbinZhang1,2*,RobertHanner2 1Department ofAquacultureLifeScience,ShanghaiOceanUniversity,Shanghai,China, 2DepartmentofIntegrativeBiology,UniversityofGuelph,Guelph,Ontario, Canada Abstract Background:DNAbarcodingisonemeansofestablishingarapid,accurate,andcost-effectivesystemfortheidentification of species. It involves the use of short, standard gene targets to create sequence profiles of known species against sequencesofunknownsthatcanbematchedandsubsequentlyidentified.TheFishBarcodeofLife(FISH-BOL)campaign hastheprimarygoalofgatheringDNAbarcoderecordsforalltheworld’sfishspecies.AsacontributiontoFISH-BOL,we examinedthedegreetowhichDNAbarcodingcandiscriminatemarinefishesfromtheSouthChinaSea. Methodology/Principal Findings: DNA barcodes of cytochrome oxidase subunit I (COI) were characterized using 1336 specimens that belong to 242 species fishes from the South ChinaSea. All specimen provenance data (including digital specimenimagesandgeospatialcoordinatesofcollectionlocalities)andcollateralsequenceinformationwereassembled using Barcode of Life Data System (BOLD; ). Small intraspecific and large interspecific differences createdistinctgeneticboundariesamongmostspecies.Inaddition,theefficiencyoftwomitochondrialgenes,16SrRNA (16S)andcytochromeb(cytb),andonenuclearribosomalgene,18SrRNA(18S),wasalsoevaluatedforafewselectgroups ofspecies. Conclusions/Significance: The present study provides evidence for the effectiveness of DNA barcoding as a tool for monitoringmarinebiodiversity.OpenaccessdataoffishesfromtheSouthChinaSeacanbenefitrelativeapplicationsin ecologyandtaxonomy. Citation: Zhang J, Hanner R (2012) Molecular Approach to the Identification of Fish in the South China Sea. PLoS ONE 7(2): e30621. doi:10.1371/ journal.pone.0030621 Editor:IndraNeilSarkar,UniversityofVermont,UnitedStatesofAmerica ReceivedFebruary26,2011;AcceptedDecember22,2011;PublishedFebruary17,2012 Copyright: ? 2012 Zhang, Hanner. T