Molecular Characterization of the Neuronatin Gene in the Porcine Placenta 英文参考文献.docVIP

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Molecular Characterization of the Neuronatin Gene in the Porcine Placenta 英文参考文献.doc

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Molecular Characterization of the Neuronatin Gene in the Porcine Placenta 英文参考文献

MolecularCharacterizationoftheNeuronatinGenein thePorcinePlacenta TingGu1,XiSu1,QuanyongZhou2,XinyunLi1,MeiYu1,YiDing1,ShuhongZhao1,ChangchunLi1* 1Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, People’s Republic of China, 2Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural sciences,Nanchang,People’sRepublicofChina Abstract Imprinted genes play important roles in placental and embryonic development. Neuronatin (NNAT), first identified as an imprintedgeneinhumanandmousebrains,playedimportantrolesinneuronaldifferentiationinthebrainandinglucose- mediated insulin secretion in pancreatic b cells. In the pig, NNAT was reported to be imprinted in eleven tissues. Our previous microarray hybridization study showed that NNAT was differentially expressed in Yorkshire and Meishan pig placentas,buttheimprintingstatusandfunctionofNNATintheplacentahavenotbeeninvestigated.Wedemonstratedfor thefirsttimethatNNATwasmonoallelicallyexpressedintheplacenta.ImmunochemistryanalysisshowedthatNNATwas locatedintheuterineluminalandglandularepitheliuminplacentas.WealsoconfirmedthedifferentialexpressionofNNAT in Meishan and Yorkshire pig placentas by qPCR. Using IPA software and the published literature, we created a model network of the possible relationships between NNAT and glucose transporter genes. A dual luciferase reporter assay demonstrated that the crucial promoter region of NNAT contained a CANNTG sequence in the +210 to +215 positions, whichcorrespondedtotheE-box.OurfindingsdemonstratedimportantrolesofNNATinplacentafunction. Citation: Gu T, Su X, Zhou Q, Li X, Yu M, et al. (2012) Molecular Characterization of the Neuronatin Gene in the Porcine Placenta. PLoS ONE 7(8): e43325. doi:10.1371/journal.pone.0043325 Editor:HuaijunZhou,UniversityofCalifornia,Davis,UnitedStatesofAmerica ReceivedApril6,2012