Molecular Characterizations of a Novel Putative DNA-Binding Protein LvDBP23 in Marine Shrimp L. vannamei Tissues and Molting Stages 英文参考文献.docVIP

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Molecular Characterizations of a Novel Putative DNA-Binding Protein LvDBP23 in Marine Shrimp L. vannamei Tissues and Molting Stages 英文参考文献.doc

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Molecular Characterizations of a Novel Putative DNA-Binding Protein LvDBP23 in Marine Shrimp L. vannamei Tissues and Molting Stages 英文参考文献

MolecularCharacterizationsofaNovelPutativeDNA- BindingProteinLvDBP23inMarineShrimpL.vannamei TissuesandMoltingStages YanisaLaoong-u-thai1,2,BaopingZhao1,AmornratPhongdara3*,JinzengYang1,4* 1Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America, 2Department of Chemical Engineering,FacultyofEngineering,BuraphaUniversity,Chonburi,Thailand,3CenterforGenomicsandBioinformaticsResearch,PrinceofSongklaUniversity,Songkhla, Thailand,4CollegeofAnimalScienceandTechnology,HuazhongAgriculturalUniversity,Wuhan,People’sRepublicofChina Abstract Background:LitopenaeusVannamei,wellknownaspacificwhiteshrimp,isthemostpopularshrimpintheworldshrimp market.Identification andcharacterization ofshrimpmuscle regulatorygenesarenotonlyimportantfor shrimpgenetic improvement,butalsofacilitatecomparativegenomictoolsforunderstandingofmuscledevelopmentandregeneration. Methodology/PrincipalFindings:AnovelmRNAencodingforaputativeDNA-bindingproteinLvDBP23wasidentifiedfrom Litopenaeus vannamei abdominal muscle cDNA library. The LvDBP23 cDNA contains 639 nucleotides of protein-coding sequencewithdeduced212aminoacidsofpredictedmolecularmass23.32kDawithglycine-richdomainataminoacid position 94–130. The mRNA sequence is successfully used for producing LvDBP23 recombinant protein in sf9 insect cell expressionsystem.TheexpressionofLvDBP23mRNAispresentedinabdominalmuscleandswimminglegmuscle,aswell asothertissuesincludingintestine,lymphoidandgill.ThemRNAexpressionhasthehighestlevelinabdominalmuscleinall tested tissues. LVDBP23 transcript during the molt cycle is highly expressed in the intermolt stage. In vitro nucleic acid- bindingassaysrevealthatLvDBP23proteincanbindtobothssDNAanddsDNA,indicatingitspossibleroleofregulationof genetranscription. Conclusions/Significance:WearethefirsttoreportaDNA-bindingproteinidentifiedfromtheabdominalmuscletissueof marineshrimpL.Vannamei.Itshigh-levelspecificexpressionduringtheintermotstagesugge