Mutations in Drosophila GreatwallScant Reveal Its Roles in Mitosis and Meiosis and Interdependence with Polo Kinase 英文参考文献.docVIP
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Mutations in Drosophila GreatwallScant Reveal Its Roles in Mitosis and Meiosis and Interdependence with Polo Kinase 英文参考文献
MutationsinDrosophilaGreatwall/Scant
RevealItsRolesinMitosisandMeiosis
andInterdependencewithPoloKinase
Vincent Archambault[,Xinbei Zhao[,Helen White-Cooper¤,Adelaide T.C.Carpenter, David M.Glover*
CRUKCellCycleGeneticsGroup,DepartmentofGenetics,UniversityofCambridge,Cambridge,UnitedKingdom
Polo is a conserved kinase that coordinates many events of mitosis and meiosis, but how it is regulated remains
unclear.Drosophilafemaleshavingonlyonewild-typealleleofthepolokinasegeneandthedominantScantmutation
produceembryosinwhichoneofthecentrosomesdetachesfromthenuclearenvelopeinlateprophase.Weshowthat
Scant creates ahyperactive form of Greatwall (Gwl) with altered specificity in vitro, another protein kinase recently
implicated in mitotic entry in Drosophila and Xenopus. Excess Gwl activity in embryos causes developmental failure
thatcanberescuedbyincreasingmaternalPolodosage,indicatingthatcoordinationbetweenthetwomitotickinases
iscrucialformitoticprogression.RevertantallelesofScantthatrestorefertilitytopolo–Scantheterozygousfemalesare
recessive alleles or deficiencies of gwl; they show chromatin condensation defects and anaphase bridges in larval
neuroblasts.OnerecessivemutantallelespecificallydisruptsaGwlisoformstronglyexpressedduringvitellogenesis.
Females hemizygous for this allele are sterile, and their oocytes fail to arrest in metaphase I of meiosis; both
homologues and sister chromatids separate on elongated meiotic spindles with little or no segregation. This allelic
seriesofgwlmutantshighlightsthemultiplerolesofGwlinbothmitoticandmeioticprogression.Ourresultsindicate
thatGwlactivityantagonizesPoloandthusidentifyanimportantregulatoryinteractionofthecellcycle.
Citation: Archambault V, Zhao X, White-Cooper H, Carpenter ATC, Glover DM (2007) Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and
interdependencewithPolokinase.PLoSGenet3(11):e200.doi:10.1371/journal.pgen.0030200
phosphorylation of cohesin subunits by Polo kinases (re-
vie
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