No Relationship between Embryo Morphology and Successful Derivation of Human Embryonic Stem Cell Lines 英文参考文献.docVIP

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No Relationship between Embryo Morphology and Successful Derivation of Human Embryonic Stem Cell Lines 英文参考文献.doc

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No Relationship between Embryo Morphology and Successful Derivation of Human Embryonic Stem Cell Lines 英文参考文献

NoRelationshipbetweenEmbryoMorphologyand SuccessfulDerivationofHumanEmbryonicStemCell Lines SusanneStro¨m1*,KennyRodriguez-Wallberg1,2,FridaHolm1,RositaBergstro¨m1,LindaEklund2 ,Anne- MarieStro¨mberg1,OutiHovatta1 1DepartmentofClinicalScience,InterventionandTechnology,KarolinskaInstitutet,Stockholm,Sweden,2FertilityUnit,KarolinskaUniversityHospital,Huddinge,Sweden Abstract Background:Thelargenumber(30)ofpermanenthumanembryonicstemcell(hESC)linesandadditional29whichdidnot continuegrowing,inourlaboratoryatKarolinskaInstitutethavegivenusapossibilitytoanalysetherelationshipbetween embryo morphology and the success of derivation of hESC lines. The derivation method has been improved during the period2002–2009,towardsfewerxeno-components.Embryoqualityisimportantasregardsthelikelihoodofpregnancy, butthereislittleinformationregardinglikelihoodofstemcellderivation. Methods: We evaluated the relationship of pronuclear zygote stage, the score based on embryo morphology and developmentalrateatcleavagestate,andthemorphologyoftheblastocystatthetimeofdonationtostemcellresearch,to seehowtheycorrelatedtosuccessfulestablishmentofnewhESClines. Results: Derivation of hESC lines succeeded from poor quality and good quality embryos in the same extent. In several blastocysts, no real inner cell mass (ICM) was seen, but permanent well growing hESC lines could be established. One tripronuclear(3PN)zygote,whichdevelopedtoblastocyststage,gaveorigintoakaryotypicallynormalhESCline. Conclusion:EvenverypoorqualityembryoswithfewcellsintheICMcangiveorigintohESClines. Citation:Stro¨mS,Rodriguez-WallbergK,HolmF,Bergstro¨mR,EklundL,etal.(2010)NoRelationshipbetweenEmbryoMorphologyandSuccessfulDerivationof HumanEmbryonicStemCellLines.PLoSONE5(12):e15329.doi:10.1371/journal.pone.0015329 Editor:ToshiShioda,MassachusettsGeneralHospital,UnitedStatesofAmerica ReceivedAugust23,2010;AcceptedNovember8,2010;PublishedDecember31,2010 Copyright: ? 2010 Stro¨m et al. This is an open-access article distributed unde