Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology 英文参考文献.docVIP

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Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology 英文参考文献.doc

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Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology 英文参考文献

Open Access Re 2009LiuetVoal.lumesea10,rchIssue 3, Article R26 Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology Tao Liu*, David Sims? and Buzz Baum* Addresses: *MRC Laboratory of Molecular Cell Biology, UCL, Gower Street, London WC1E 6BT, UK. ?The Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK. Correspondence: Buzz Baum. Email: b.baum@ucl.ac.uk Published: 5 March 2009 Received: 27 November 2008 Revised: 18 February 2009 Accepted: 5 March 2009 Genome Biology 2009, 10:R26 (doi:10.1186/gb-2009-10-3-r26) The electronic version of this article is the complete one and can be found online at /2009/10/3/R26 ? 2009 Liu et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. RegulationinpcluPdairnagllaelofnRecellNurAonimorphologynterferenceal function fscreensor the kandinasegeneminexpressionibrain/DYRarraysK1A ininthsixe reDrosophilagulation ofcellprolinestrusioidentifiedn morphoregulatorslogy./pof cell morphology, Abstract Background: In recent years RNAi screening has proven a powerful tool for dissecting gene functions in animal cells in culture. However, to date, most RNAi screens have been performed in a single cell line, and results then extrapolated across cell types and systems. Results: Here, to dissect generic and cell type-specific mechanisms underlying cell morphology, we have performed identical kinome RNAi screens in six different Drosophila cell lines, derived from two distinct tissues of origin. This analysis identified a core set of kinases required for normal cell morphology in all lines tested, together with a number of kinases with cell typ