Preserved MHC Class II Antigen Processing in Monocytes from HIV-Infected Individuals 英文参考文献.docVIP

下载本文档

2
0
约2.54万字
约 7页
2017-05-12 发布于上海
举报
版权申诉

Preserved MHC Class II Antigen Processing in Monocytes from HIV-Infected Individuals 英文参考文献.doc

1、本文档共7页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Preserved MHC Class II Antigen Processing in Monocytes from HIV-Infected Individuals 英文参考文献

PreservedMHCClassIIAntigenProcessinginMonocytes fromHIV-InfectedIndividuals LailaWoc-Colburn1,LaviniaSmultea1,LakshmiRamachandra2,DavidH.Canaday1,3* 1Division of Infectious Diseases and HIV Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America, 2Department of Pathology,CaseWesternReserveUniversitySchoolofMedicine,Cleveland,Ohio,UnitedStatesofAmerica,3GeriatricResearch,Education,andClinicalCenter,Cleveland VeteransAffairs,Cleveland,Ohio,UnitedStatesofAmerica Abstract Background: MHC-II restricted CD4+ T cells are dependent on antigen presenting cells (APC) for their activation. APC dysfunction in HIV-infected individuals could accelerate or exacerbate CD4+ T cell dysfunction and may contribute to increased levels of immunodeficiency seen in some patients regardless of their CD4+ T cell numbers. Here we test the hypothesisthatAPCfromHIV-infectedindividualshavediminishedantigenprocessingandpresentationcapacity. Methodology/Principal Findings: Monocytes (MN) were purified by immuno-magnetic bead isolation techniques from HLA-DR1.01+orDR15.01+HIV-infectedanduninfectedindividuals.MNwereanalyzedforsurfaceMHC-IIexpressionandfor antigen processing and presentation capacity after overnight incubation with soluble antigen or peptide and HLA-DR matchedTcellhybridomas.SurfaceexpressionofHLA-DRwas20%reduced(p,0.03)onMNfromHIV-infectedindividuals. In spite of this, there was no significant difference in antigen processing and presentation by MN from 14 HIV-infected donors(8HLA-DR1.01+and6HLA-DR15.01+)comparedto24HIV-uninfectedHLA-matchedsubjects. Conclusions/Significance:WedemonstratedthatMHCclassIIantigenprocessingandpresentationispreservedinMNfrom HIV-infectedindividuals.ThisfurthersupportstheconceptthatthisaspectofAPCfunctiondoesnotfurthercontributeto CD4+TcelldysfunctioninHIVdisease. Citation: Woc-Colburn L, Smultea L, Ramachandra L, Canaday DH (2010) Preserved MHC Class II Antigen Processing in Monocytes from HIV-Infected In