Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics 英文参考文献.docVIP

Molecules 2012, 17, 5972-5987; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics Yan Chen *, Qingqing Wu, Zhenghai Zhang, Ling Yuan, Xuan Liu and Lei Zhou Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medical, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China; E-Mails: wqq18314@126.com (Q.W.); davidpharm@ (Z.Z.); yuanl.china@ (L.Y.); liu_xuan1987@163.com (X.L.); youleiwuheng@ (L.Z.) * Author to whom correspondence should be addressed; E-Mail:ychen202@; Tel.: +86-25-8560-8672; Fax: +86-25-8563-7809. Received: 9 April 2012; in revised form: 8 May 2012 / Accepted: 9 May 2012 / Published: 18 May 2012 Abstract: This study aimed to investigate the in vitro skin permeation and in vivo antineoplastic effect of curcumin by using liposomes as the transdermal drug-delivery system. Soybean phospholipids (SPC), egg yolk phospholipids (EPC), and hydrogenated soybean phospholipids (HSPC) were selected for the preparation of different kinds of phospholipids composed of curcumin-loaded liposomes: C-SPC-L (curcumin-loaded SPC liposomes), C-EPC-L (curcumin-loaded EPC liposomes), and C-HSPC-L (curcumin-loaded HSPC liposomes). The physical properties of different lipsomes were investigated as follows: photon correlation spectroscopy revealed that the average particle sizes of the three types of curcumin-loaded liposomes were 82.37 ± 2.19 nm (C-SPC-L), 83.13 ± 4.89 nm (C-EPC-L), and 92.42 ± 4.56 nm (C-HSPC-L), respectively. The encapsulation efficiency values were found to be 82.32 ± 3.91%, 81.59 ± 2.38%, and 80.77 ± 4.12%, respectively. An in vitro skin penetration study indicated that C-SPC-L most significantly promoted drug permeation and deposition followed by C-EPC-L, C-HSPC-L, and curcumin solution. Moreover, C-SPC-L di