Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA 英文参考文献.docVIP

Sensors 2007, 7, 708-718 sensors ISSN 1424-8220 ? 2007 by MDPI /sensors Full Research Paper Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA Chunhua Liu 1, Xiupei Yang 1,3, Hongyan Yuan 2, Zaide Zhou 1 and Dan Xiao 1,2* 1 College of Chemistry, Sichuan University, Chengdu 610064, P. R. China 2 College of Chemical Engineering, Sichuan University, Chengdu 610064, P. R. China 3 College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, P. R. China * Author to whom correspondence should be addressed; Fax +86 28 Tel. +86 28 E-mail: xiaodan@ Received: 16 March 2007 / Accepted: 29 May 2007 / Published: 31 May 2007 Abstract: A new method to prepare silver nanoparticles was reported in this paper, which based on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. The proposed method is effective, rapid, and convenient. Transmission electron microscope (TEM) and X-ray diffraction (XRD) were used to characterize the structure of the particles, respectively. It was found that the average diameter of the particles was about 10 nm and the shape was spherical. And the strong fluorescence signal of the silver nanoparticles solution can be quenched after the adding of the calf thymus DNA (ct-DNA) solution. The quenched fluorescence intensity was linear with the concentration of ct-DNA in the range of 0.5 to 5.0 × 104 ng mL-1 with a detection limit of 0.3 ng mL-1. Keywords: silver, nanoparticle, fluorescence, calf thymus DNA (ct-DNA) 1. Introduction In recent years, noble metal nanoparticles have been extensively studied and various approaches have been employed for the preparation of metal nanoparticles [1-6]. Among the noble metal nanoparticles, silver nanoparticles have attracted more attention [7-13] for their advantage on various studies such as photosensitive components [14], catalysts [15-17], and surface-enhan