Prevalence and Infection Load Dynamics of Rickettsia felis in Actively Feeding Cat Fleas 英文参考文献.docVIP

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Prevalence and Infection Load Dynamics of Rickettsia felis in Actively Feeding Cat Fleas 英文参考文献.doc

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Prevalence and Infection Load Dynamics of Rickettsia felis in Actively Feeding Cat Fleas 英文参考文献

PrevalenceandInfectionLoadDynamicsofRickettsia felisinActivelyFeedingCatFleas KathrynE.Reif1,RhettW.Stout1,GretchenC.Henry1,LaneD.Foil2,KevinR.Macaluso1* 1DepartmentofPathobiologicalSciences,LouisianaStateUniversity,BatonRouge,Louisiana,UnitedStatesofAmerica,2DepartmentofBiologicalSciences,Louisiana StateUniversity,BatonRouge,Louisiana,UnitedStatesofAmerica Abstract Background:Rickettsiafelisisaflea-associatedrickettsialpathogenrecurrentlyidentifiedinbothcolonizedandwild-caught catfleas,Ctenocephalidesfelis.Wehypothesizedthatwithincolonizedfleas,theintimaterelationshipbetweenR.felisandC. felisallowsforthecoordinationofrickettsialreplicationandmetabolicallyactiveperiodsduringfleabloodmealacquisition andoogenesis. Methodology/PrincipalFindings:Aquantitativereal-timePCRassaywasdevelopedtoquantifyR.felisinactivelyfeedingR. felis-infectedfleas.Inthreeseparatetrials,fleaswereallowedtofeedoncats,andameanof3.96106R.felis17-kDagene copieswasdetectedforeachflea.AdistinctR.felisinfectionpatternwasnotobservedinfleasduringnineconsecutivedays ofbloodfeeding.However,aninversecorrelationbetweentheprevalenceofR.felis-infection,whichrangedfrom96%in Trial1to35%inTrial3,andtheR.felis-infectionloadinindividualfleaswasidentified.ExpressionofR.felis-infectionloadas aratioofR.felis/C.felisgenesconfirmedthatfleasinTrial3hadsignificantlygreaterrickettsialloadsthanthoseinTrial1. Conclusion/Significance: Examining rickettsial infection dynamics in the flea vector will further elucidate the intimate relationshipbetweenR.felisandC.felis,andfacilitateamoreaccurateunderstandingoftheecologyandepidemiologyofR. felistransmissioninnature. Citation:ReifKE,StoutRW,HenryGC,FoilLD,MacalusoKR(2008)PrevalenceandInfectionLoadDynamicsofRickettsiafelisinActivelyFeedingCatFleas.PLoS ONE3(7):e2805.doi:10.1371/journal.pone.0002805 Editor:FloydRomesberg,TheScrippsResearchInstitute,UnitedStatesofAmerica ReceivedApril2,2008;AcceptedJune20,2008;PublishedJuly30,2008 Copyright:?2008Reifetal.Thisisanopen-accessarticle