Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China 英文参考文献.docVIP

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Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China 英文参考文献.doc

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Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China 英文参考文献

Acta Veterinaria Scandinavica BioMedCentral Research Open Access Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China Yijia Yan?, Wen Zhang?, Quan Shen, Li Cui and Xiuguo Hua* Address: Shanghai Key laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, PR China Email: Yijia Yan - y_yan11@; Wen Zhang - z0216wen@; Quan Shen - Shenquanfly@; Li Cui - lcui@; Xiuguo Hua* - hxg@ * Corresponding author ?Equal contributors Published: 31 May 2008 Received: 19 January 2008 Accepted: 31 May 2008 Acta Veterinaria Scandinavica 2008, 50:12 doi:10.1186/1751-0147-50-12 This article is available from: /content/50/1/12 ? 2008 Yan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Hepatitis E virus (HEV) is a zoonotic pathogen of which swine was reported as major reservoirs. HEV has been divided into 4 different genotypes according to phylogenetic analysis. Recent reports showed that genotype 4 HEV is freely transmitted between humans and swine in eastern China, including Shanghai area. This paper investigated the recent infection status of HEV among swine population of Shanghai area in China. Methods: 480 swine faecal specimens were collected from 23 farms which distribute all over Shanghai from September to November, 2007 and tested for the presence of HEV RNA by the polymerase chain reaction (PCR). Results: Our results showed that 26.1% (6/23) of the swine farms were positive for HEV RNA and the positive rate of the six farms were ranged from 9.1% to 33.3%. The HEV RNA positive rate for total samples were 5% (24/480). The resulte