Production and Processing of siRNA Precursor Transcripts from the Highly Repetitive Maize Genome 英文参考文献.docVIP
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Production and Processing of siRNA Precursor Transcripts from the Highly Repetitive Maize Genome 英文参考文献
ProductionandProcessingofsiRNAPrecursor
TranscriptsfromtheHighlyRepetitiveMaizeGenome
ChristopherJ.Hale,KarlF.ErhardJr.,DamonLisch,JayB.Hollick*
DepartmentofPlantandMicrobialBiology,UniversityofCaliforniaBerkeley,Berkeley,California,UnitedStatesofAmerica
Abstract
Mutations affecting the maintenance of heritable epigenetic states in maize identify multiple RNA–directed DNA
methylation (RdDM) factors including RMR1, a novel member of a plant-specific clade of Snf2-related proteins. Here we
showthatRMR1isnecessaryfortheaccumulationofamajorityof24ntsmallRNAs,includingthosederivedfromLong-
TerminalRepeat(LTR)retrotransposons,themostcommonrepetitivefeatureinthemaizegenome.Ageneticanalysisof
DNA transposon repression indicates that RMR1 acts upstream of the RNA–dependent RNA polymerase, RDR2 (MOP1).
Surprisingly,weshowthatnon-polyadenylatedtranscriptsfromasamplingofLTRretrotransposonsarelostinbothrmr1
andrdr2mutants.Incontrast,plantsdeficientforRNAPolymeraseIV(PolIV)functionshowanincreaseinpolyadenylated
LTRRNAtranscripts.ThesefindingssupportamodelinwhichPolIVfunctionsindependentlyofthesmallRNAaccumulation
facilitatedbyRMR1andRDR2andsupportthatalossofPolIVleadstoRNAPolymeraseII–basedtranscription.Additionally,
thelackofchangesingeneralgenomehomeostasisinrmr1mutants,despitethegloballossof24ntsmallRNAs,challenges
theperceivedrolesofsiRNAsinmaintainingfunctionalheterochromatininthegenomesofoutcrossinggrassspecies.
Citation:HaleCJ,ErhardKFJr,LischD,HollickJB(2009)ProductionandProcessingofsiRNAPrecursorTranscriptsfromtheHighlyRepetitiveMaizeGenome.PLoS
Genet5(8):e1000598.doi:10.1371/journal.pgen.1000598
Editor:GregoryP.Copenhaver,TheUniversityofNorthCarolinaatChapelHill,UnitedStatesofAmerica
ReceivedApril14,2009;AcceptedJuly14,2009;PublishedAugust14,2009
Copyright:?2009Haleetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted
use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredit
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