Production of haploids and doubled haploids in oil palm 英文参考文献.docVIP

Production of haploids and doubled haploids in oil palm 英文参考文献.doc

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Production of haploids and doubled haploids in oil palm 英文参考文献

Dunwelletal.BMCPlantBiology2010,10:218 /1471-2229/10/218 RESEARCH ARTICLE OpenAccess Productionofhaploidsanddoubledhaploidsin oilpalm JimMDunwell1*?,MikeJWilkinson2*?,StephenNelson3,SriWening4,AndrewCSitorus4,DeviMienanti4, YuzerAlfiko4,AdamECroxford2,CarolineSFord2,BrianPForster5,PeterDSCaligari3,5,6 Abstract Background:Oilpalmistheworld’smostproductiveoil-foodcropdespiteyieldingwellbelowitstheoretical maximum.ThismaximumcouldbeapproachedwiththeintroductionofeliteF1varieties.Thedevelopmentof suchelitelineshasthusfarbeenpreventedbydifficultiesingeneratinghomozygousparentaltypesforF1 generation. Results:Herewepresentthefirsthigh-throughputscreentoidentifyspontaneously-formedhaploid(H)and doubledhaploid(DH)palms.Wesecuredover1,000HsandoneDHfromgeneticallydiversematerialandderived furtherDH/mixoploidpalmsfromHsusingcolchicine.WedemonstratedviabilityofpollenfromHplantsand expecttogenerate100%homogeneousF1seedfromintercrossesbetweenDH/mixoploidsoncetheydevelop femaleinflorescences. Conclusions:ThisstudyhasgeneratedgeneticallydiverseH/DHpalmsfromwhichparentalclonescanbeselected insufficientnumberstoenablethecommercial-scalebreedingofF1varieties.Theanticipatedstepincreasein productivitymayhelptorelievepressuretoextendpalmcultivation,andlimitfurtherexpansionintobiodiverse rainforest. Background Desire for a more generic H/DH production system to SuccessofearlyF1hybridmaizevarietiesexemplifiesthe improve agricultural yields is increasing as population advantagesofheterosis[1].Theuseofdoubledhaploids growth,climatechange,biofueldemandandotherland- as parents for F1 variety production fully exploits this use pressures intensify. Clearly, in any species the phenomenonandhasenabledsubstantialyieldimprove- productionofF1varietiesdependsnotonlyonthepro- mentsinseveral crops[2,3]. Thisstrategy wasoutlined ductionofhomozygouslinestoactasparents,butalso withthefirstDHcropvariety[4]andhasledtoH/DH itrequiresanefficientmethodtointercrosstheparents. produ

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