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Production of haploids and doubled haploids in oil palm 英文参考文献
Dunwelletal.BMCPlantBiology2010,10:218
/1471-2229/10/218
RESEARCH ARTICLE
OpenAccess
Productionofhaploidsanddoubledhaploidsin
oilpalm
JimMDunwell1*?,MikeJWilkinson2*?,StephenNelson3,SriWening4,AndrewCSitorus4,DeviMienanti4,
YuzerAlfiko4,AdamECroxford2,CarolineSFord2,BrianPForster5,PeterDSCaligari3,5,6
Abstract
Background:Oilpalmistheworld’smostproductiveoil-foodcropdespiteyieldingwellbelowitstheoretical
maximum.ThismaximumcouldbeapproachedwiththeintroductionofeliteF1varieties.Thedevelopmentof
suchelitelineshasthusfarbeenpreventedbydifficultiesingeneratinghomozygousparentaltypesforF1
generation.
Results:Herewepresentthefirsthigh-throughputscreentoidentifyspontaneously-formedhaploid(H)and
doubledhaploid(DH)palms.Wesecuredover1,000HsandoneDHfromgeneticallydiversematerialandderived
furtherDH/mixoploidpalmsfromHsusingcolchicine.WedemonstratedviabilityofpollenfromHplantsand
expecttogenerate100%homogeneousF1seedfromintercrossesbetweenDH/mixoploidsoncetheydevelop
femaleinflorescences.
Conclusions:ThisstudyhasgeneratedgeneticallydiverseH/DHpalmsfromwhichparentalclonescanbeselected
insufficientnumberstoenablethecommercial-scalebreedingofF1varieties.Theanticipatedstepincreasein
productivitymayhelptorelievepressuretoextendpalmcultivation,andlimitfurtherexpansionintobiodiverse
rainforest.
Background
Desire for a more generic H/DH production system to
SuccessofearlyF1hybridmaizevarietiesexemplifiesthe improve agricultural yields is increasing as population
advantagesofheterosis[1].Theuseofdoubledhaploids growth,climatechange,biofueldemandandotherland-
as parents for F1 variety production fully exploits this use pressures intensify. Clearly, in any species the
phenomenonandhasenabledsubstantialyieldimprove- productionofF1varietiesdependsnotonlyonthepro-
mentsinseveral crops[2,3]. Thisstrategy wasoutlined ductionofhomozygouslinestoactasparents,butalso
withthefirstDHcropvariety[4]andhasledtoH/DH itrequiresanefficientmethodtointercrosstheparents.
produ
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