Prostatic Acid Phosphatase Is Expressed in Peptidergic and Nonpeptidergic Nociceptive Neurons of Mice and Rats 英文参考文献.docVIP

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Prostatic Acid Phosphatase Is Expressed in Peptidergic and Nonpeptidergic Nociceptive Neurons of Mice and Rats 英文参考文献.doc

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Prostatic Acid Phosphatase Is Expressed in Peptidergic and Nonpeptidergic Nociceptive Neurons of Mice and Rats 英文参考文献

ProstaticAcidPhosphataseIsExpressedinPeptidergic andNonpeptidergicNociceptiveNeuronsofMiceand Rats BonnieTaylor-Blake,MarkJ.Zylka* DepartmentofCellandMolecularPhysiology,UNCNeuroscienceCenter,UniversityofNorthCarolina,ChapelHill,NorthCarolina,UnitedStatesofAmerica Abstract Thiaminemonophosphatase(TMPase,alsoknownasFluoride-resistantacidphosphataseorFRAP)isaclassichistochemical marker of small- to medium-diameter dorsal root ganglia (DRG) neurons and has primarily been studied in the rat. Previously,wefoundthatTMPasewasmolecularlyidenticaltoProstaticacidphosphatase(PAP)usingmice.Inaddition,PAP + wasexpressedinamajorityofnonpeptidergic,isolectinB4-binding(IB4 )nociceptiveneuronsandasubsetofpeptidergic, calcitoningene-relatedpeptide-containing(CGRP+)nociceptiveneurons.Atthetime,wewereunabletodetermineifPAP waspresentinratDRGneuronsbecausetheantibodyweuseddidnotcross-reactwithPAPinrattissues.Inourpresent study, we generated a chicken polyclonal antibody against the secretory isoform of mouse PAP. This antibody detects mouse,ratandhumanPAPproteinonwesternblots.Additionally,thisantibodydetectsPAPinmouseandratsmall-to + medium-diameterDRGneuronsandaxonterminalsinlaminaIIofspinalcord.Intherat,92.5%ofallPAP cellsbindthe nonpeptidergic marker IB4 and 31.8% of all PAP cells contain the peptidergic marker CGRP. Although PAP is found in peptidergic and nonpeptidergic neurons of mice and rats, the percentage of PAP neurons that express these markers + + differs between species. Moreover, PAP+ axon terminals in the rat partially overlap with Protein kinase Cc (PKCc+) interneurons in dorsal spinal cord whereas PAP axon terminals in the mouse terminate dorsal to PKCc interneurons. Collectively,ourstudieshighlightsimilaritiesanddifferencesinPAPlocalizationwithinnociceptiveneuronsofmiceandrats. + + Citation:Taylor-BlakeB,ZylkaMJ(2010)ProstaticAcidPhosphataseIsExpressedinPeptidergicandNonpeptidergicNociceptiveNeuronsofMiceandRats.PLoS ONE5(1):e8674.doi:10.1371/journal.