Proton nuclear magnetic resonance spectroscopy based investigation on propylene glycol toxicosis in a Holstein cow 英文参考文献.docVIP

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Proton nuclear magnetic resonance spectroscopy based investigation on propylene glycol toxicosis in a Holstein cow 英文参考文献.doc

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Proton nuclear magnetic resonance spectroscopy based investigation on propylene glycol toxicosis in a Holstein cow 英文参考文献

Acta Veterinaria Scandinavica BioMedCentral Research Open Access Proton nuclear magnetic resonance spectroscopy based investigation on propylene glycol toxicosis in a Holstein cow Hanne Christine Bertram*1, Bent Ole Petersen2, Jens ? Duus2, Mogens Larsen3, Birgitte-Marie L Raun3 and Niels Bastian Kristensen3 Address: 1Department of Food Science, Faculty of Agricultural Sciences, Aarhus University, P.O. Box 102, DK-5792 ?rslev, Denmark, 2Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark and 3Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, Aarhus University, P.O. Box 50, DK-8830 Tjele, Denmark Email: Hanne Christine Bertram* - HanneC.Bertram@agrsci.dk; Bent Ole Petersen - bp@crc.dk; Jens ? Duus - jd@crc.dk; Mogens Larsen - Mogens.Larsen@agrsci.dk; Birgitte-Marie L Raun - Birgitte.Raun@agrsci.dk; Niels Bastian Kristensen - NielsB.Kristensen@agrsci.dk * Corresponding author Published: 13 June 2009 Received: 19 March 2009 Accepted: 13 June 2009 Acta Veterinaria Scandinavica 2009, 51:25 doi:10.1186/1751-0147-51-25 This article is available from: /content/51/1/25 ? 2009 Bertram et al., licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: It is unknown which metabolites are responsible for propylene glycol (PG)-induced toxicosis, and a better understanding of the underlying mechanisms explaining incidences of abnormal behaviour of dairy cows fed PG is therefore needed. Methods: The study included three cows of which one developed PG toxicosis. In order to investigate how the metabolism of PG differed in the cow developing toxicosis, proton nuclear magnetic resonance (NMR) spectroscopy was applied on ruminal fluids and blood pla