Proximity of Transmembrane Segments 5 and 8 of the Glutamate Transporter GLT-1 Inferred from Paired Cysteine Mutagenesis 英文参考文献.docVIP
- 1、本文档共7页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Proximity of Transmembrane Segments 5 and 8 of the Glutamate Transporter GLT-1 Inferred from Paired Cysteine Mutagenesis 英文参考文献
ProximityofTransmembraneSegments5and8ofthe
GlutamateTransporterGLT-1InferredfromPaired
CysteineMutagenesis
XiupingZhang1,ShaogangQu2*
1China-America Cancer Research Institute, Guangdong Medical College, Dongguan, Guangdong, China, 2Department of Immunology, Southern Medical University,
Guangzhou,Guangdong,China
Abstract
Background: GLT-1 is a glial glutamate transporter which maintains low synaptic concentrations of the excitatory
neurotransmitterenablingefficientsynaptictransmission.BasedonthecrystalstructureofthebacterialhomologueGltPh ,it
hasbeenproposedthatthereentrantloopHP2,whichconnectstransmembranedomains(TM)7and8,movestoopenand
closeaccesstothebindingpocketfromtheextracellularmedium.HowevertheconformationchangebetweenTM5and
TM8duringthetransportcycleisnotclearyet.Weusedpairedcysteinemutagenesisinconjunctionwithtreatmentswith
Copper(II)(1,10-Phenanthroline)3(CuPh),toverifythepredictedproximityofresidueslocatedatthesestructuralelementsof
GLT-1.
Methodology/Principal Findings: To assess the proximity of transmembrane domain (TM) 5 relative to TM8 during
transportbytheglialglutamatetransporterGLT-1/EAAT2,cysteinepairswereintroducedattheextracellularendsofthese
structural elements. A complete inhibition of transport by Copper(II)(1,10-Phenanthroline)3 is observed in the double
mutants I295C/I463C and G297C/I463C, but not in the corresponding single mutants. Glutamate and potassium, both
expectedtoincreasetheproportionofinward-facingtransporters,significantlyprotectedagainsttheinhibitionoftransport
activityofI295C/I463CandG297C/I463CbyCuPh.TransportbythedoublemutantsI295C/I463CandG297C/I463Calsowas
2+
inhibitedbyCd .
Conclusions/Significance: Our results suggest that TM5 (Ile-295, Gly-297) is in close proximity to TM8 (Ile-463) in the
mammaliantransporter,andthatthespatialrelationshipbetweenthesedomainsisalteredduringthetransportcycle.
Citation: Zhang X, Qu S (2011) Proximity of Transmembrane Segments 5 and 8 of the Glutamate Transporter GLT-1 Inferred from Pa
您可能关注的文档
- Protein-Bound Uremic Toxins New Insight from Clinical Studies 英文参考文献.doc
- Protein X of Hepatitis B Virus Origin and Structure Similarity with the Central Domain of DNA Glycosylase 英文参考文献.doc
- Proteinase-3 as the major autoantigen of c-ANCA is strongly expressed in lung tissue of patients with Wegener's granulomatosis 英文参考文献.doc
- Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice 英文参考文献.doc
- Protein-losing enteropathy in patients with Fontan circulation is it triggered by infection 英文参考文献.doc
- Protein Secretion Is Required for Pregnancy-Associated Plasma Protein-A to Promote Lung Cancer Growth In Vivo 英文参考文献.doc
- Protein-Protein Interaction Analysis Highlights Additional Loci of Interest for Multiple Sclerosis 英文参考文献.doc
- Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3 英文参考文献.doc
- Proteins from Avastin? (bevacizumab) Show Tyrosine Nitrations for which the Consequences Are Completely Unclear 英文参考文献.doc
- Proteinopathy-induced neuronal senescence a hypothesis for brain failure in Alzheimer's and other neurodegenerative diseases 英文参考文献.doc
- 2026届高考数学总复习(第1轮)基础版第50讲 直线的方程.pptx
- 《关于组织开展能源领域氢能试点工作的通知》解读及对策.docx
- 二年级数学奇妙的动物世界跨学科项目化学习设计.docx
- 高一美术传承与创新中国近现代美术思政融合课教学设计.docx
- 2025年湖北省省直辖行政单位选调生考试(行政职业能力测验)综合能力测试题带答案.docx
- 2025年温州医科大学仁济学院单招语文测试模拟题库附答案.docx
- 2025年清远职业技术学院单招(语文)测试模拟题库附答案.docx
- 2025年湖北省咸宁市事业单位招聘考试(职业能力倾向测验)题库及答案1套.docx
- 2025年温州大学单招语文测试题库有答案.docx
- 2025年湖北省荆州市单招语文测试模拟题库a4版.docx
文档评论(0)