Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2TC7 intestinal cells 英文参考文献.docVIP

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Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2TC7 intestinal cells 英文参考文献.doc

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Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2TC7 intestinal cells 英文参考文献

Madietal.GutPathogens2010,2:16 /content/2/1/16 RESEARCH OpenAccess Pseudomonasfluorescensaltersepithelial permeabilityandtranslocatesacrossCaco-2/TC7 intestinalcells AmarMadi1,PascalSvinareff2,NicoleOrange1,MarcGJFeuilloley1,NathalieConnil1* Abstract Background:Pseudomonasfluorescenshaslongbeenconsideredasapsychrotrophicmicroorganism.Recently,we haveshownthatclinicalstrainsofP.fluorescens(biovar1)areabletoadaptatagrowthtemperatureof37°Cor aboveandinduceaspecificinflammatoryresponse.Interestingly,ahighlyspecificantigenofP.fluorescens,I2,is detectedintheserumofpatientswithCrohn’sdiseasebutthepossibleroleofthisbacteriuminthediseasehas notyetbeenexplored.Inthepresentstudy,weexaminedtheabilityofapsychrotrophicandaclinicalstrainof P.fluorescenstomodulatethepermeabilityofaCaco-2/TC7intestinalepithelialmodel,reorganizetheactin cytoskeleton,invadethetargetcellsandtranslocateacrosstheepithelium.Thebehaviourofthesetwostrainswas comparedtothatofthewellknownopportunisticpathogenP.aeruginosaPAO1. Results:BothstrainsofP.fluorescenswerefoundtodecreasethetransepithelialresistance(TER)ofCaco-2/TC7 differentiatedmonolayers.ThiswasassociatedwithanincreaseinparacellularpermeabilityandF-actin microfilamentsrearrangements.Moreover,theinvasionandtranslocationtestsdemonstratedthatthetwostrains usedinthisstudycaninvadeandtranslocateacrossthedifferentiatedCaco-2/TC7cellmonolayers. Conclusions:Thepresentworkshowsforthefirsttime,thatP.fluorescensisabletoaltertheintestinalepithelial barrierfunctionbydisorganizingtheF-actinmicrofilamentnetwork.Moreover,werevealthatindependentlyof theirorigins,thetwoP.fluorescensstrainscantranslocateacrossdifferentiatedCaco-2/TC7cellmonolayersbyusing thetranscellularpathway.Thesefindingscould,atleastinpart,explainthepresenceoftheP.fluorescensspecificI2 antigenintheserumofpatientswithCrohn’ sdisease. Background intestinalcells[3].Theseobservationsandtheincidence Pseudomonasfluorescens has longbeenconsideredas a of declared nosocomial infections due to this germ [