Rapid analysis of formic acid, acetic acid, and furfural in pretreated wheat straw hydrolysates and ethanol in a bioethanol fermentation using atmospheric pressure chemical ionisation mass spectrometry 英文参考文献.docVIP

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Rapid analysis of formic acid, acetic acid, and furfural in pretreated wheat straw hydrolysates and ethanol in a bioethanol fermentation using atmospheric pressure chemical ionisation mass spectrometry 英文参考文献.doc

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Rapid analysis of formic acid, acetic acid, and furfural in pretreated wheat straw hydrolysates and ethanol in a bioethanol fermentation using atmospheric pressure chemical ionisation mass spectrometry 英文参考文献

Daviesetal.BiotechnologyforBiofuels2011,4:28 /content/4/1/28 METHODOLOGY OpenAccess Rapidanalysisofformicacid,aceticacid,and furfuralinpretreatedwheatstrawhydrolysates andethanolinabioethanolfermentationusing atmosphericpressurechemicalionisationmass spectrometry ScottMDavies,RobSLinforth*,StuartJWilkinson,KatherineASmartandDavidJCook Abstract Atmosphericpressurechemicalionisationmassspectrometry(APCI-MS)offersadvantagesasarapidanalytical techniqueforthequantificationofthreebiomassdegradationproducts(aceticacid,formicacidandfurfural)within pretreatedwheatstrawhydrolysatesandtheanalysisofethanolduringfermentation.Thedataweobtainedusing APCI-MScorrelatedsignificantlywithhigh-performanceliquidchromatographyanalysiswhilstofferingtheanalyst minimalsamplepreparationandfastersamplethroughput. Background [2,3].Highlevelsofweakorganicacidsleadtoacidifica- Conversion of lignocellulosic biomass into bioethanol tion of the yeast cytosol. This cytosolic acidification currentlyinvolvesfourmainprocesses:physicochemical depletestheintracellularATPpoolthroughadiversion pretreatment, enzymatic saccharification, fermentation ofATPtoplasmamembraneATPases,whichpumpout anddistillation and/orpurification ofethanol.Pretreat- H+ ionstoregulate theintracellular pH[2]. Furansare ment of lignocellulosic biomass is necessary to disrupt believed to inhibit key fermentative enzymes such as the inherently recalcitrant plant cell wall structure to alcohol dehydrogenase, pyruvate dehydrogenase and facilitateenzymeaccessandthushydrolysisofconstitu- aldehyde dehydrogenase [2]. Acetic acid in biomass ent polysaccharides to sugars in preparation for hydrolysates is derived primarily from deacetylation of fermentation. thehemicellulose xylansidechains. Formicacidmean- Currentpretreatmentprocessesemployhightempera- whilearisesfromthedegradationoffurfuralor5-hydro- turesand/orextremepHlevelstodisruptandfacilitate xymethylfurfural(HMF),bothofwhicharederivedfrom theseparation