Rapid and Sensitive Detection of Plesiomonas shigelloides by Loop-Mediated Isothermal Amplification of the hugA Gene 英文参考文献.docVIP

下载本文档

8
0
约2.64万字
约 6页
2017-05-13 发布于上海
举报
版权申诉

Rapid and Sensitive Detection of Plesiomonas shigelloides by Loop-Mediated Isothermal Amplification of the hugA Gene 英文参考文献.doc

1、本文档共6页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Rapid and Sensitive Detection of Plesiomonas shigelloides by Loop-Mediated Isothermal Amplification of the hugA Gene 英文参考文献

RapidandSensitiveDetectionofPlesiomonas shigelloidesbyLoop-MediatedIsothermalAmplification ofthehugAGene ShuangMeng,JianguoXu,YanwenXiong,ChangyunYe* State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, ChineseCenter for Disease ControlandPrevention,Changping,Beijing,People’sRepublicofChina Abstract Plesiomonas shigelloides is one of the causative agents of human gastroenteritis, with increasing number of reports describingsuchinfectionsinrecentyears.Inthisstudy,thehugAgenewaschosenasthetargettodesignloop-mediated isothermalamplification(LAMP)assaysfortherapid,specific,andsensitivedetectionofP.shigelloides.Theperformanceof the assay with reference plasmids and spiked human stools as samples was evaluated and compared with those of quantitativePCR(qPCR).Nofalse-positiveresultswereobservedforthe32non-P.shigelloidesstrainsusedtoevaluateassay specificity. The limit of detection for P. shigelloides was approximately 20 copies per reaction in reference plasmids and 56103CFUpergraminspikedhumanstool,whichweremoresensitivethantheresultsofqPCR.Whenappliedinhuman stoolsamplesspikedwith2lowlevelsofP.shigelloides,theLAMPassaysachievedaccuratedetectionafter6-henrichment. Inconclusion,theLAMPassaydevelopedinthisstudyisavaluablemethodforrapid,cost-effective,andsimpledetectionof P.shigelloidesinbasicclinicalandfieldlaboratoriesintheruralareasofChina. Citation:MengS,XuJ,XiongY,YeC(2012)RapidandSensitiveDetectionofPlesiomonasshigelloidesbyLoop-MediatedIsothermalAmplificationofthehugA Gene.PLoSONE7(10):e41978.doi:10.1371/journal.pone.0041978 Editor:StefanBereswill,Charite′-University MedicineBerlin,Germany ReceivedMay30,2012;AcceptedJune27,2012;PublishedOctober15,2012 Copyright: ? 2012 Meng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsour