Rectification of the Water Permeability in COS-7 Cells at 22, 10 and 0°C 英文参考文献.docVIP

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Rectification of the Water Permeability in COS-7 Cells at 22, 10 and 0°C 英文参考文献.doc

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Rectification of the Water Permeability in COS-7 Cells at 22, 10 and 0°C 英文参考文献

RectificationoftheWaterPermeabilityinCOS-7Cellsat 6 22,10and0 C DianaB.Peckys1,3*,F.W.Kleinhans2,3,PeterMazur3 1DepartmentofMolecularPhysiologyandBiophysics,VanderbiltUniversitySchoolofMedicine,Nashville,Tennessee,UnitedStatesofAmerica,2DepartmentofPhysics, IndianaUniversity-PurdueUniversityatIndianapolis,Indianapolis,Indiana,UnitedStatesofAmerica,3DepartmentofBiochemistry,andCellularandMolecularBiology, UniversityofTennessee,Knoxville,Tennessee,UnitedStatesofAmerica Abstract The osmotic and permeability parameters of a cell membrane are essential physico-chemical properties of a cell and particularly important with respect to cell volume changes and the regulation thereof. Here, we report the hydraulic conductivity,Lp,thenon-osmoticvolume,Vb,andtheArrheniusactivationenergy,Ea,ofmammalianCOS-7cells.Theratio ofVbtotheisotoniccellvolume,Vc iso,was0.29.Ea,theactivationenergyrequiredforthepermeationofwaterthroughthe cellmembrane,was10,700,and12,000cal/molunderhyper-andhypotonicconditions,respectively.AveragevaluesforLp were calculated from swell/shrink curves by using an integrated equation for Lp. The curves represented the volume changesof358individuallymeasuredcells,placedintosolutionsofnonpermeatingsolutesof157or602mOsm/kg(at0,10 or 22uC) and imaged over time. Lp estimates for all six combinations of osmolality and temperature were calculated, resulting in values of 0.11, 0.21, and 0.10mm/min/atm for exosmotic flow and 0.79, 1.73 and 1.87mm/min/atm for endosmoticflow(at0,10and22uC,respectively).TheunexpectedfindingofseveralfoldhigherLpvaluesforendosmotic flowindicateshighlyasymmetricmembranepermeabilityforwaterinCOS-7.Thisphenomenonisknownasrectification andhasmainlybeenreportedforplantcell,butonlyrarelyforanimalcells.Althoughthemechanismunderlyingthestrong rectification found in COS-7 cells is yet unknown, it is a phenomenon of biological interest and has important practical consequences,forinstance,inthedevelopmentofoptimalcryopreservation. Citation:PeckysD