miR-451下调MIF的表达并抑制其促鼻咽癌细胞浸润和转移的作用.pdfVIP

现代肿瘤医学 2016 年02 月 第24 卷第04 期 MODERN ONCOLOGY ,Feb. 2016 ,VOL 24 , NO. 04 .527. miR -451 下调 MIF 的表达并抑制其促鼻咽癌细胞授润和转移的作用 刘展艳l 气赵子潇1 ，易 纯l miR - 451 downregulates the expression of macrophage migration inhibitory 阳ctor ( MIF) and inhibits the effection that MIF promo悔s the invasion and meta剖asis in naso- pharyngeal carcinoma cells 1 l Liu Zhanyan 1 立， Zhao Zixiao ,Yi Chun 1Department 01 Pathology , Chinese Medicine 01Hunan Univers町， Hunan Changsha 410208 , China ;2 Department 01 Oncology , The Æ庐lia­ ted Hospital 01 Hu阳n Academy 01 Traditωnal Chinese Medicine ,Hunan Changsha 41仪邸， China. [ Abstract 1 Objective :To study the role of migration inhibitory factor macrophage (MIF) in the invasion and me- tastasis in nasopharyngeal carcinoma cells. Methods: The westem blot was used to measure the expression level of MIF in HONEl ,5 - 8F ,CNE - 1 , CNE - 2 cell lines and the adhesion protein expression level in HONEl cell lines with overexpressed of MIF and the effect of miR - 451 on the expression of MIF in 5 - 8F and 293A cell lines. The Transwell experiment was used to test the invasion and metastasis ability in nasopharyngeal carcinoma cell lines. The impact of miR - 451 on MIF was verified by fluorescence reporting system. Results:币le amount of MIF was increased in 5 -8F which w植 high transfer nasopharyngeal carcinoma cellline. MIF could decrease the adhesion protein expres- sion level and the intercellular adhesion and increase the invasion and metastasis in nasopharyngeal carcinoma cells. The expression of MIF was regulated by miR - 451 ,which could inhibit the expression of MIF. CoocI皿ioo: MIF plays an important role in the invasion an