血虚证红细胞免疫功能和变形性,淋巴细胞转化功能及自 ….pdfVIP

． 卜 999年4月弟6卷第4 ·151· · 论著 · 血虚证红细胞免疫功能和变形性、淋巴细胞 转化功能及自由基代谢的研究凸 陈如采 梁 毂 欧 敏 方碧琴 摘要 目的：探讨血虚证免疫功能变化和自由基代谢的特点及其意义。方法：选择34例临床血虚证患者及 30例正常人，并采用缓慢放血及饥饱失宜联合方法制作24只血虚证家兔模型。对血虚证红细胞C。b受体 (RBC—C，bR)花环率．红细胞免疫复合物(RBC—IC)花环率、红细胞变形指数(DI)、T淋巴细胞亚群、T和B淋 巴细胞转化率、红细胞超氧化物歧化酶(SOD)和过氧化脂质(LPO)的变化进行了研究；同时观察中药四物溺对 上述指标的影响。结果：血虚证时，RBC—CsbR花环率明显降低．RBC—IC花环率明显升高；红细胞DI值降低 T淋巴细胞亚群OKT 和OKT．明显降低．OKT。略高但变化不明显，OKT．／OKTa明显下降{T、B淋巴细胞转 化率均明显降低,SOD活性明显降低，LPO含量明显升高。P0．05或P0．01。给血虚证家兔服用四物汤后， 其RBC—CabR明显提高．RBC—IC明显降低{红细胞DI值明显升高；T、B淋巴细胞转化率明显增加；SOD活性 明显上升，LPO含量显著下降。P0．05或P0．01。结论：血虚证时细胞免疫功能明显降低．红细胞变形性下 降+机体产生自由基。四物溺具有提高RBC功能、改善血虚症状、促进免疫功能恢复和消除自由基的作用。 关簟调 血虚 证候 红细胞免疫 自由基 红细胞变形性 淋巴细胞转俐功能 一 一 — — ～ — — — — — Studies oB~rythrocytic immune funetio and deformabfllttes，lymphocytic transformable fuactions．and metabolisms of free radicals ia blood deficiency syndrome Chen R~qua．．Liang Yi．Ou Min+et a1．The A班 ated Hospital of Hubei College TCM+Wu,han 430061 Abstract Objective：To investigate the changes in immune functions．the metabolic characteristics and significances of free radicals in blood deficiency syndrome(BDS)．Metheds：Clinically 34 cases with BDS and 3O normal subjects were selected．Simuhaneously 24 models of rabbits with BDS were constructed by slowly draw— ing blood and irregular feeding．The changes in red blood cell(RBC)一C3b receptor rosette rate+RBC—immune complex(IC)rosette rate·deformable index of RBC(DI)，T lymphocytic subgroups．transforma ble rates of T and B lymphocytes·erythrocytic superoxide dismutase(SOD)