Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection 英文参考文献.docVIP

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Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection 英文参考文献.doc

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Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection 英文参考文献

RapidandTargetedIntrogressionofGenesintoPopular WheatCultivarsUsingMarker-AssistedBackground Selection HarpinderS.Randhawa1.,JasdeepS.Mutti1.,KimKidwell1,CraigF.Morris2,XianmingChen3, KulvinderS.Gill1* 1DepartmentofCropandSoilSciences,WashingtonStateUniversity,Pullman,Washington,UnitedStatesofAmerica,2USDA-ARSWesternWheatQualityLaboratory, Washington State University, Pullman, Washington, United States of America, 3USDA-ARS, Wheat Genetics, Quality, Physiology, and Disease Research Unit and DepartmentofPlantPathology,WashingtonStateUniversity,Pullman,Washington,UnitedStatesofAmerica Abstract A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized,where97%ormoreofarecurrentparentgenome(RPG)canberecoveredinjusttwobackcross(BC)generations. A four-step MABS method was developed based on ‘Plabsim’ computer simulations and wheat genome structure information.Duringempiricaloptimizationofthismethod,doublerecombinantsaroundthetargetgenewereselectedina step-wise fashion during the two BC cycles followed by selection for recurrent parent genotype on non-carrier chromosomes. The average spacing between carrier chromosome markers was ,4cM. For non-carrier chromosome markersthatflankedeachofthe48wheatgene-richregions,thisdistancewas,12cM.Employedtointrogressseedling striperust(Pucciniastriiformisf.sp.tritici)resistancegeneYr15intothespringwheatcultivar‘Zak’,markeranalysisof2,187 backcross-derivedprogenyresultedintherecoveryofaBC2F2:3plantwith97%oftherecurrentparentgenome.Incontrast, only82%oftherecurrentparentgenomewasrecoveredinphenotypicallyselectedBC4F7plantsdevelopedwithoutMABS. Fieldevaluationresultsfrom17locationsindicatedthattheMABS-derivedlinewaseitherequalorsuperiortotherecurrent parent for the tested agronomic characteristics. Based on these results, MABS is recommended as a strategy for rapidly introgressingatargetedgeneintoawheatgenotypeinjusttwobackcrossgenerationswhilerecovering97%ormoreofthe r