Simplified Automated Image Analysis for Detection and Phenotyping of Mycobacterium tuberculosis on Porous Supports by Monitoring Growing Microcolonies 英文参考文献.docVIP

下载本文档

5
0
约3.28万字
约 8页
2017-05-13 发布于上海
举报
版权申诉

Simplified Automated Image Analysis for Detection and Phenotyping of Mycobacterium tuberculosis on Porous Supports by Monitoring Growing Microcolonies 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Simplified Automated Image Analysis for Detection and Phenotyping of Mycobacterium tuberculosis on Porous Supports by Monitoring Growing Microcolonies 英文参考文献

SimplifiedAutomatedImageAnalysisforDetectionand PhenotypingofMycobacteriumtuberculosisonPorous SupportsbyMonitoringGrowingMicrocolonies AliceL.denHertog1*,DennisW.Visser2,ColinJ.Ingham3,FrankH.A.G.Fey4,PaulR.Klatser1,RichardM. Anthony1 1KITBiomedicalResearch,RoyalTropicalInstitute,Amsterdam,TheNetherlands,2Vleuten,TheNetherlands,3MicrodishBV,Houten,TheNetherlands,4CCMCentrefor ConceptsinMechatronics,Nuenen,TheNetherlands Abstract Background: Even with the advent of nucleic acid (NA) amplification technologies the culture of mycobacteria for diagnosticandotherapplicationsremainsofcriticalimportance.Notablymicroscopicobserveddrugsusceptibilitytesting (MODS),asopposedtotraditionalcultureonsolidmediaorautomatedliquidculture,hasshownpotentialtobothspeedup andincreasetheprovisionofmycobacterialcultureinhighburdensettings. Methods: Here we explore the growth of Mycobacterial tuberculosis microcolonies, imaged by automated digital microscopy, cultured on a porous aluminium oxide (PAO) supports. Repeated imaging during colony growth greatly simplifies‘‘computervision’’andpresumptive identificationofmicrocolonies wasachievedhereusingexistingpublically availablealgorithms.Oursystemthusallowsthegrowthofindividualmicrocoloniestobemonitoredandcritically,alsoto changethemediaduringthegrowthphasewithoutdisruptingthemicrocolonies.Transferofidentifiedmicrocoloniesonto selective media allowed us, within 1-2 bacterial generations, to rapidly detect the drug susceptibility of individual microcolonies,eliminatingtheneedfortimeconsumingsubculturingortheinoculationofmultipleparallelcultures. Significance: Monitoring the phenotype of individual microcolonies as they grow has immense potential for research, screening, and ultimately M. tuberculosis diagnostic applications. The method described is particularly appealing with respecttospeedandautomation. Citation: den Hertog AL, Visser DW, Ingham CJ, Fey FHAG, Klatser PR, et al. (2010) Simplified Automated Image Analysis for Detection and