Simple and Rapid Method for the Determination of Uric Acid-Independent Antioxidant Capacity 英文参考文献.docVIP

Molecules 2011, 16, 7058-7067; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Simple and Rapid Method for the Determination of Uric Acid-Independent Antioxidant Capacity Darko Duplancic 1, Lea Kukoc-Modun 2,*, Darko Modun 3 and Njegomir Radic 2 1 Department of Cardiology, University Hospital Split, Soltanska 1, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia; E-Mail: darko.duplancic@ (D.D.) 2 Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Teslina 10/V, 21000 Split, Croatia; E-Mail: njegomir.radic@ (N.R.) 3 Department of Pharmacology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia; E-Mail: drmodun@ (D.M.) * Author to whom correspondence should be addressed; E-Mail: lkmodun@; Tel.: +38521329463; Fax: +38521329461. Received: 22 July 2011; in revised form: 11 August 2011 / Accepted: 15 August 2011 / Published: 17 August 2011 Abstract: Determination of the relative contribution of uric acid level increases to the total measured antioxidative activity could be very useful for testing antioxidative products and their effect on human health. The aim of this report is to present a simple spectrophotometric method that combines the measurement of total antioxidative capacity of a sample by ferric reducing/antioxidative power (FRAP) assay, with the uricase-reaction (specific elimination of uric acid), in order to establish and correct for the contribution of uric acid in FRAP values. We measured FRAP values, with (uric acid-independent antioxidant capacity, TAC-UA) and without (total antioxidant capacity, TAC) uricase treatment, and expressed it as μmol/L of uric acid equivalents. In such way, it was possible to determine both total and uric acid-independent antioxidant capacity, plasma uric acid (UA, as the difference between TAC and TAC-UA)