Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue 英文参考文献.docVIP

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Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue 英文参考文献.doc

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Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue 英文参考文献

Simple,Time-SavingDyeStainingofProteinsforSodium DodecylSulfate–PolyacrylamideGelElectrophoresis UsingCoomassieBlue Wei-HuaDong,Tian-YunWang*,FangWang,Jun-HeZhang DepartmentofBiochemistryandMolecularBiology,XinxiangMedicalUniversity,Xinxiang,Henan,China Abstract A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassieblueisdescribed.Theprotocolcomprisesstainingandquickwashingsteps,whichcanbecompletedin0.5h.It hasasensitivityof10ng,comparablewiththatofconventionalCoomassieBrilliantBlueGstainingwithphosphoricacidin thestainingsolution.Inaddition,thedyestaindoesnotcontainanyamountofacidandmethanol,suchasphosphoricacid. Consideringthespeed,simplicity,andlowcost,thedyestainmaybeofmorepracticalvaluethanotherdye-basedprotein stainsinroutineproteomicresearch. Citation: Dong W-H, Wang T-Y, Wang F, Zhang J-H (2011) Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel ElectrophoresisUsingCoomassieBlue.PLoSONE6(8):e22394.doi:10.1371/journal.pone.0022394 Editor:VladimirN.Uversky,UniversityofSouthFlorida,UnitedStatesofAmerica ReceivedMay18,2011;AcceptedJune20,2011;PublishedAugust5,2011 Thisisanopen-accessarticle,freeofallcopyright,andmaybefreelyreproduced,distributed,transmitted,modified,builtupon,orotherwiseusedbyanyonefor anylawfulpurpose.TheworkismadeavailableundertheCreativeCommonsCC0publicdomaindedication. Funding:ThisworkwassupportedbytheNationalNaturalScienceFoundationC010202),8/portal/Proj_List.asp.Thefundershad noroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:wtianyuncn@126.com Introduction quantitativeanalysisthansilverstaining,andthecapacitytoallow possible modifications for fast or highly sensitive staining. Coomassie stain has become the key compound in Blue native PAGEinthelast10years[13,14].However,itstillrequiresalong sta