Simple Sequence Repeat Polymorphisms (SSRPs) for Evaluation of Molecular Diversity and Germplasm Classification of Minor Crops 英文参考文献.docVIP

Molecules 2009, 14, 4546-4569; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review Simple Sequence Repeat Polymorphisms (SSRPs) for Evaluation of Molecular Diversity and Germplasm Classification of Minor Crops Yong-Jin Park 1, Ju Kyong Lee 2 and Nam-Soo Kim 3,4,* 1 Department of Plant Sciences, Kongju National University, Yesan 340-702, Korea 2 Division of Bio-Resources Technology, Kangwon National University, Chunchon 200-701, Korea 3 Department of Molecular Biosciences, Kangwon National University, Chunchon 200-701, Korea 4 Institute of Biosciences and Biotechnology, Kangwon National University, Chunchon 200-701, Korea * Author to whom correspondence should be addressed; E-Mail: kimnamsu@kangwon.ac.kr. Received: 13 September 2009; in revised form: 5 November 2009 / Accepted: 10 November 2009 / Published: 10 November 2009 Abstract: Evaluation of the genetic diversity among populations is an essential prerequisite for the preservation of endangered species. Thousands of new accessions are introduced into germplasm institutes each year, thereby necessitating assessment of their molecular diversity before elimination of the redundant genotypes. Of the protocols that facilitate the assessment of molecular diversity, SSRPs (simple sequence repeat polymorphisms) or microsatellite variation is the preferred system since it detects a large number of DNA polymorphisms with relatively simple technical complexity. The paucity of information on DNA sequences has limited their widespread utilization in the assessment of genetic diversity of minor or neglected crop species. However, recent advancements in DNA sequencing and PCR technologies in conjunction with sophisticated computer software have facilitated the development of SSRP markers in minor crops. This review examines the development and molecular nature of SSR markers