Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals 英文参考文献.docVIP

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Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals 英文参考文献.doc

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Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals 英文参考文献

Sensors2011,11,10557-10570;doi:10.3390/s111110557 OPENACCESS sensors ISSN1424-8220 /journal/sensors Article SimplisticAttachmentandMultispectralImagingwith SemiconductorNanocrystals TravisL.Jennings ,RobertC.Triulzi,GuoliangTao,ZacharyE.St.Louisand ? SaraG.Becker-Catania ? eBioscience,Inc.,10255ScienceCenterDrive,SanDiego,CA92121,USA; E-Mails:robert.triulzi@(R.C.T.);guoliang.tao@(G.T.); soccerrox03@(Z.E.S.L.) ? Authortowhomcorrespondenceshouldbeaddressed; E-Mails:travis.jennings@(T.L.J.); sara.becker-catania@(S.G.B.-C.);Tel.:+858-784-5390(T.L.J.). Received:14September2011;inrevisedform:26October2011/Accepted:26October2011/ Published:7November2011 Abstract:Advancesinspectraldeconvolutiontechnologiesarerapidlyenablingresearchers toreplaceorenhancetraditionalepi?uorescencemicroscopeswithinstrumentscapableof detectingnumerousmarkerssimultaneouslyinamultiplexedfashion. Whilesigni?cantly expeditingsamplethroughputandelucidatingsampleinformation,thistechnologyislimited bythespectralwidthofcommon?uorescencereporters.Semiconductornanocrystals(NC’s) are very bright, narrow band ?uorescence emitters with great potential for multiplexed ?uorescencedetection,howevertheavailabilityofNC’swithfacileattachmentchemistries totargetingmoleculeshasbeenaseverelimitationtotheadvancementofNCtechnology inapplicationssuchasimmunocytochemistryandimmunohistochemistry. Herewereport thedevelopmentofsimple,yetnovelattachmentchemistriesforantibodiesontoNC’sand demonstrate how spectral deconvolution technology enables the multiplexed detection of 5distinctNC-antibodyconjugateswith?uorescenceemissionwavelengthsseparatedbyas littleas20nm. Keywords: quantum dot; nanocrystal; IHC; immunohistochemistry; spectral imaging; multiplexing;conjugation;antibody Sensors2011,11 10558 1.Introduction Immunohistochemistry (IHC), or the immunological staining and detection of cellular markers in tissue, is a mainstay technique in pathology and diagnostics research and is the current recipient of several