Sperm morphology in Estonian and Tori Breed Stallions 英文参考文献.docVIP

Acta vet. scand. 2004 , 45, 11-18. Sperm morphology in Estonian and Tori Breed Stallions By A. Kavak1,3, N. Lundeheim2, M. Aidnik1, S.Einarsson3 1Department of O bstetrics and Gynaecology, Faculty of Veterinary Medicine, Estonian Agricultural University, Tartu, Estonia, 2Department of Animal Breeding and Genetics, Faculty of Agriculture, Landscape Planning and Horticulture, and 3 Department of O bstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish Uni- versity of Agricultural Sciences, CRU, Uppsala, Sweden. Kavak A, Lundeheim N, Aidnik M, Einarsson S: Sperm morphology in Estonian and Tori Breed stallions. Acta vet. scand. 2004, 45, 11-18. – The standard procedure for assessing the breeding potential of a stallion includes the parameter total number of spermatozoa classi?ed as morphologically normal. This study investigated sperm mor- phology of fresh semen in randomly chosen Estonian (E, n=8) and Tori (T, n=7) breed stallions with proven fertility. Two ejaculates were examined from each stallion. An aliquot from each ejaculate was ?xed in 1 mL formol-saline immediately after collec- tion and examined with phase-contrast microscope at a magni?cation 1000x for all types of morphological abnormalities. Furthermore smears were prepared and stained according to Williams (carbolfuchsin-eosin) for a more detailed examination of the sperm heads with light microscope at a magni?cation 1000x. Analysis of variance was applied to the data, and results are presented as LSmeans (±SE). O ne T stallion that had a disturbance in the spermatogenesis and one 22-year-old E stallion were not included in the analyses. The T stallions had on average 57.5±4 .1% and the E-stallions 74 .4 ±3 .8% morphologically normal spermatozoa (p=0.012). In 4 of 7 T stallions and 7 of 8 E stallions both ejaculates had 50% morphologically normal spermatozoa. There was a signi?cant difference between breeds in mean percentage of proximal