Specific Strains of Escherichia coli Are Pathogenic for the Endometrium of Cattle and Cause Pelvic Inflammatory Disease in Cattle and Mice 英文参考文献.docVIP

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Specific Strains of Escherichia coli Are Pathogenic for the Endometrium of Cattle and Cause Pelvic Inflammatory Disease in Cattle and Mice 英文参考文献.doc

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Specific Strains of Escherichia coli Are Pathogenic for the Endometrium of Cattle and Cause Pelvic Inflammatory Disease in Cattle and Mice 英文参考文献

SpecificStrainsofEscherichiacoliArePathogenicforthe EndometriumofCattleandCausePelvicInflammatory DiseaseinCattleandMice I.MartinSheldon1*,AndrewN.Rycroft2,BelginDogan3,MelanieCraven3,JohnJ.Bromfield1 ,Alyssa Chandler3,MarkH.Roberts1,SianB.Price2,RobertO.Gilbert3,KennethW.Simpson3 1InstituteofLifeScience,SchoolofMedicine,SwanseaUniversity,SingletonPark,Swansea,UnitedKingdom,2RoyalVeterinaryCollege,Hatfield,Hertfordshire,United Kingdom,3DepartmentofClinicalSciences,CollegeofVeterinaryMedicine,CornellUniversity,Ithaca,NewYork,UnitedStatesofAmerica Abstract Background:Escherichiacoliarewidespreadintheenvironmentandpathogenicstrainscausediseasesofmucosalsurfaces includingthefemalegenitaltract.Pelvicinflammatorydisease(PID;metritis)orendometritisaffects,40%ofcattleafter parturition. We tested the expectation that multiple genetically diverse E. coli from the environment opportunistically contaminatetheuterinelumenafterparturitiontoestablishPID. Methodology/PrincipalFindings:DistinctclonalgroupsofE.coliwereidentifiedbyRandomAmplificationofPolymorphic DNA (RAPD) and Multilocus sequence typing (MLST) from animals with uterine disease and these differed from known diarrhoeic or extra-intestinal pathogenic E. coli. The endometrial pathogenic E. coli (EnPEC) were more adherent and invasiveforendometrialepithelialandstromalcells,comparedwithE.coliisolatedfromtheuterusofclinicallyunaffected animals. The endometrial epithelial and stromal cells produced more prostaglandin E2 and interleukin-8 in response to lipopolysaccharide(LPS)purifiedfromEnPECcomparedwithnon-pathogenicE.coli.TheEnPECortheirLPSalsocausedPID wheninfusedintotheuterusofmicewithaccumulationofneutrophilsandmacrophagesintheendometrium.Infusionof EnPEC was only associated with bacterial invasion of theendometrium and myometrium. Despite their ability to invade culturedcells,elicithostcellresponsesandestablishPID,EnPEClackedsixteengenescommonlyassociatedwithadhesion andinvasionbyentericorextraintestinalpathogenicE.c