Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use 英文参考文献.docVIP

Molecules 2012, 17, 11669-11679; doi:10.3390/molecules171011669 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use Jixiang Lai 1, Can Xin 2, Ya Zhao 3, Bing Feng 1, Congfen He 4, Yinmao Dong 4, Yun Fang 1 and Shaomin Wei 1,3, * 1 School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China School of Chemical Engineering, Hebei United University, Tangshan 063009, Hebei, China Shanghai Jahwa United Co. Ltd, Shanghai 200082, China 2 3 4 School of Science, Beijing Technology and Business University, Beijing 100048, China * Author to whom correspondence should be addressed; E-Mail: weishaomin@; Tel.: +86-021-2501-2302; Fax: +86-021-6976-8460. Received: 10 July 2012; in revised form: 13 September 2012 / Accepted: 19 September 2012 / Published: 1 October 2012 Abstract: Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified by H2O2 were studied using an MTT colorimetric assay. The safety of the extracts was determined using the red blood cell (RBC) test, chick chorioallantoic membrane (CAM) assay and human patch test. Results show that DPPH radical scavenging rates at different shoot lengths were all greater than 95%, while the tyrosinase inhibition capacity of the extracts reached 98%. Hemolysis rate in all extracts were lower than 10%, below the 20% regulatory limit for the RBC test. No signs of allergic reactions were observed in the human patch t