The upregulation of thiamine (vitamin B1) biosynthesis in Arabidopsis thaliana seedlings under salt and osmotic stress conditions is mediated by abscisic acid at the early stages of this stress response 英文参考文献.docVIP

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The upregulation of thiamine (vitamin B1) biosynthesis in Arabidopsis thaliana seedlings under salt and osmotic stress conditions is mediated by abscisic acid at the early stages of this stress response 英文参考文献.doc

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The upregulation of thiamine (vitamin B1) biosynthesis in Arabidopsis thaliana seedlings under salt and osmotic stress conditions is mediated by abscisic acid at the early stages of this stress response 英文参考文献

Rapala-Koziketal.BMCPlantBiology2012,12:2 /1471-2229/12/2 RESEARCH ARTICLE OpenAccess Theupregulationofthiamine(vitaminB1) biosynthesisinArabidopsisthalianaseedlings undersaltandosmoticstressconditionsis mediatedbyabscisicacidattheearlystagesof thisstressresponse MariaRapala-Kozik1*,NataliaWolak1,MartaKujda1andAgnieszkaKBanas2 Abstract Background:RecentreportssuggestthatvitaminB1(thiamine)participatesintheprocessesunderlyingplant adaptationstocertaintypesofabioticandbioticstress,mainlyoxidativestress.Mostofthegenescodingfor enzymesinvolvedinthiaminebiosynthesisinArabidopsisthalianahavebeenidentified.Inourpresentstudy,we examinedtheexpressionofthiaminebiosyntheticgenes,ofgenesencodingthiaminediphosphate-dependent enzymesandthelevelsofthiaminecompoundsduringtheearly(sensing)andlate(adaptation)responsesof Arabidopsisseedlingstooxidative,salinityandosmoticstress.Thepossiblerolesofplanthormonesinthe regulationofthethiaminecontributiontostressresponseswerealsoexplored. Results:TheexpressionofArabidopsisgenesinvolvedinthethiaminediphosphatebiosynthesispathway, includingthatofTHI1,THIC,TH1andTPK,wasanalyzedfor48hinseedlingssubjectedtoNaClorsorbitol treatment.Thesegeneswerefoundtobepredominantlyup-regulatedintheearlyphase(2-6h)ofthestress response.Thechangesinthesegenetranscriptlevelswerefurtherfoundtocorrelatewithincreasesinthiamine anditsdiphosphateestercontentinseedlings,aswellaswiththeenhancementofgeneexpressionforenzymes whichrequirethiaminediphosphateasacofactor,mainlya-ketoglutaratedehydrogenase,pyruvatedehydrogenase andtransketolase.Inthecaseofthephytohormonesincludingthesalicylic,jasmonicandabscisicacidswhichare knowntobeinvolvedinplantstressresponses,onlyabscisicacidwasfoundtosignificantlyinfluencethe expressionofthiaminebiosyntheticgenes,thethiaminediphosphatelevels,aswellastheexpressionofgenes codingformainthiaminediphosphate-dependentenzymes.UsingArabidopsismutantplantsdefectiveinabscisic acidproduction,wedemonstratethatthisphytohormoneisimportantintheregulat