Three New Myrsinol Diterpenes from Euphorbia prolifera and Their Neuroprotective Activities 英文参考文献.docVIP

Molecules 2012, 17, 9520-9528; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Three New Myrsinol Diterpenes from Euphorbia prolifera and Their Neuroprotective Activities Jing Xu 1, Daqing Jin 2, Ping Guo 3, Chunfeng Xie 1, Lingzhi Fang 1 and Yuanqiang Guo 1,4,* 1 State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Molecular Drug Research, College of Pharmacy, Nankai University, Tianjin 300071, China 2 School of Medicine, Nankai University, Tianjin 300071, China 3 Department of Analytical Chemistry, College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China 4 State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China * Author to whom correspondence should be addressed; E-Mail: victgyq@163.com; Tel./Fax: +86-22-2350-2595. Received: 9 July 2012; in revised form: 25 July 2012 / Accepted: 26 July 2012 / Published: 9 August 2012 Abstract: Three new myrsinol diterpenes were isolated from the roots of Euphorbia prolifera. Their structures were elucidated as 2α-O-isobutyryl-3β,5α,7β,10,15β- penta-O-acetyl-14α-O-benzoyl-10,18-dihydromyrsinol (1), 2α-O-isobutyryl-3β-O-propion- yl-5α,7β,10,15β-tetra-O-acetyl-10,18-dihydromyrsinol (2), and 2α,14α-di-O-benzoyl- 3β,5α,7β,10,15β-penta-O-acetyl-10,18-dihydromyrsinol (3) on the basis of spectroscopic data analyses (IR, ESI-MS, HR-ESI-MS, and 1D and 2D NMR). Their neuroprotective activities were evaluated and compounds 1 and 2 showed neuroprotective effects against MPP+-induced neuronal cell death in SH-SY5Y cells. Keywords: Euphorbia prolifera; diterpenoids; myrsinol diterpenes; neuroprotective activities 1. Introduction Euphorbia prolifera Buch-Ham, belonging to the family Euphorbiaceae, is a perennial herbaceous plant distributed i