Topochemical distribution of lignin and hydroxycinnamic acids in sugar-cane cell walls and its correlation with the enzymatic hydrolysis of polysaccharides 英文参考文献.docVIP

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Topochemical distribution of lignin and hydroxycinnamic acids in sugar-cane cell walls and its correlation with the enzymatic hydrolysis of polysaccharides 英文参考文献.doc

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Topochemical distribution of lignin and hydroxycinnamic acids in sugar-cane cell walls and its correlation with the enzymatic hydrolysis of polysaccharides 英文参考文献

Siqueiraetal.BiotechnologyforBiofuels2011,4:7 /content/4/1/7 RESEARCH OpenAccess Topochemicaldistributionofligninand hydroxycinnamicacidsinsugar-canecellwalls anditscorrelationwiththeenzymatichydrolysis ofpolysaccharides GermanoSiqueira1,AdrianeMFMilagres1,WalterCarvalho1,GeraldKoch2,AndréFerraz1* Abstract Background:Ligninandhemicellulosesarethemajorcomponentslimitingenzymeinfiltrationintocellwalls. Determinationofthetopochemicaldistributionofligninandaromaticsinsugarcanemightprovideimportant dataontherecalcitranceofspecificcells.Weusedcellularultraviolet(UV)microspectrophotometry(UMSP)to topochemicallydetectligninandhydroxycinnamicacidsinindividualfiber,vesselandparenchymacellwallsof untreatedandchlorite-treatedsugarcane.Internodes,presentingtypicalvascularbundlesandsucrose-storing parenchymacells,weredividedintorindandpithfractions. Results:Vascularbundlesweremoreabundantintherind,whereasparenchymacellspredominatedinthepithregion. UVmeasurementsofuntreatedfibercellwallsgaveabsorbancespectratypicalofgrasslignin,withabandat278nm andapronouncedshoulderat315nm,assignedtothepresenceofhydroxycinnamicacidslinkedtoligninand/orto arabino-methylglucurono-xylans.Thecellwallsofvesselshadthehighestleveloflignification,followedbythoseof fibersandparenchyma.Pithparenchymacellwallswerecharacterizedbyverylowabsorbancevaluesat278nm; however,adistinctpeakat315nmindicatedthatpithparenchymacellsarenotextensivelylignified,butcontain significantamountsofhydroxycinnamicacids.CellularUVimageprofilesscannedwithanabsorbanceintensity maximumof278nmidentifiedthepatternoflignindistributionintheindividualcellwalls,withthehighest concentrationoccurringinthemiddlelamellaandcellcorners.Chloritetreatmentcausedarapidremovalof hydroxycinnamicacidsfromparenchymacellwalls,whereasthethickerfibercellwallsweredelignifiedonlyafteralong treatmentduration(4hours).Untreatedpithsampleswerepromptlyhydrolyzedbycellulases,reaching63%ofcellulose conversionafter72hoursofhydrolysis,whereasuntreatedrindsamplesachi