医学授课ppt蓝黑模板之微生物学Medical Microbiology Systemic Bactreiology多图展示，适于授课.ppt

Medical MicrobiologyLecture 3 Dr. Saleh M Y OTH PhD Medical Molecular Biotechnology and Infectious Diseases 02/10/2010 IMS - MSU STREPTOCOCCI Words must to be known Group A streptococcus (S. pyogenes) Lancefield groups Hemolysis (alpha, beta, gamma) Bacitracin susceptibility test M, T, R proteins Streptolysins O and S F protein/lipoteichoic acid Rheumatic fever/carditis/arthritis Glomerulonephritis Scarlet fever Toxic shock-like syndrome/bacteremia “Flesh-eating bacteria” Pyrogenic toxin Erythrogenic toxin Group B streptococcus (S.agalactiae) Neonatal septicemia/meningitis CAMP test Hippurate hydrolysis test Group D streptococcus Urinary tract infection/ endocarditis Bile-esculin test Enterococci Non-enterococci Large colony Minute colony Viridans streptococci Dental caries/endocarditis Streptococcus pyogenesDuring the infection, this bacteria produce pus - One of the most important pathogens - Gram positive cocci in chains - Lancefield Serological Group A - Beta Hemolytic on blood agar Hemolysis on Blood Agar Plates Beta hemolysis-organisms excretes potent hemoysins which completely lyse RBCs (complete hemolysis) thus a clear zone appears around colony. S. pyogenes Alpha hemolysis-organism excretes hemolysins which partially break down rbc (incomplete hemolysis) thus a greenish zone appears around colony. S. pneumoniae Groupable streptococci 1- A, B and D frequent 2- C, G, F less frequent Non-groupable 1- S. pneumoniae pneumonia 2- viridans streptococci e.g. S. mutans dental caries hemolysis reaction - sheep blood agar a- (beta-β) complete clearing A and B b- (alpha-α) partial hemolysis green color c- (gamma-δ) no lysis Hemolysis 1- Groups A an B β-hemolysis 2- Group D α or δ-hemolysis 3- S. pneumoniae and S. viridans α-hemolysis Group A streptococcus (S. pyogenes) S. pyogenes –suppurative 1- non-invasive - pharyngitis - skin infect