肿瘤疫苗与肿瘤主动免.pptVIP

目前DC 的临床研究已相当广泛，在黑色素瘤、卵巢癌、肾癌、乳腺癌、前列腺癌、淋巴瘤及各种血液性肿瘤的研究中已经取得了令人鼓舞的结果。 DC治疗肿瘤的临床应用 * 链亲和素标记的细胞因子修饰生物素化的肿瘤细胞疫苗 链亲和素(SA Streptavidin，SA)是由亲和素链霉菌产生的非糖基化同源四聚体蛋白。 * Tetramer analysis of antigen-specific CD8+ T cells. Although some interaction of T cells with MHC dimers can be detected, reproducible staining is best obtained with tetramers. These tetramers must be formed in solution before the addition of T cells — adding fluorescent streptavidin after the incubation of cells with monomers does not lead to cell labeling. The tetramers themselves might bind together to form higher-order oligomers. * * GM-CSF/TNFα膜表面修饰RM-1细胞疫苗治疗前列腺癌 GM-CSF可促进DC细胞成熟，并诱导巨噬细胞释TNFα。 TNFα具有改变血管的通透性，也能促使DC细胞的成熟。 * 疫苗制备 GM-CSF/TNFα 共同锚定修饰 生物素化 30%酒精 灭活 制备RM-1 细胞悬液 联合锚定细胞疫苗 * SA-GM-CSF/SA-TNFα同时锚定于RM-1细胞膜 ? ? Yin W, He Q, Hu Z ,et al, Vaccine. 2010 Jul 12;28(31):4937-44. * 联合锚定疫苗显著延长正位前列腺癌小鼠的生存期 Yin W, He Q, Hu Z ,et al, Vaccine. 2010 Jul 12;28(31):4937-44. * 联合锚定疫苗抗肿瘤免疫反应机制 联合锚定疫苗刺激脾细胞分泌IFNγ,IL-4 联合锚定疫苗显著性增加脾CD4+、CD8+T细胞的总数 Yin W, He Q, Hu Z ,et al, Vaccine. 2010 Jul 12;28(31):4937-44. * 结论 SA-hTNFα/SA-mGM-CSF膜修饰肿瘤细胞疫苗能同时锚定SA-mGM-CSF 和SA- hTNFα ，并且保持hTNFα和mGM-CSF活性。 联合锚定疫苗有效地抑制前列腺癌的生长。 Yin W, He Q, Hu Z ,et al, Vaccine. 2010 Jul 12;28(31):4937-44. * A novel immunotherapy for superficial bladder cancer by intravesical immobilization of GM-CSF Streptavidin (SA) can bind up to four D-biotin molecules SA can bind to any biotin-linked molecule rapidly and irreversibly biotin can be incorporated easily into various biological materials. Tumor cell Biotin streptavidin GM-CSF * Prepared of SA-GM-CSF fusion protein Hu Z, et al... J Cell Mol Med,2010，14：1836－1844 * Persistence of SA-GM-CSF bifunctional fusion protein immobilized on the biontinylated mucosal surface Hu Z, et al... J Cell Mol Med,2010，14：1836－1844 * Therapeutic efficacy of SA-GM-CSF in the orthotopic mouse model of MB49 bladder cancer Hu Z, et al... J Cell Mol Med,2010，14：1836－1844 * 新上市的肿瘤疫苗 ?Corixa公司和AVAX技术公司治疗黑素瘤的癌