养殖水体氨氮降解菌的分离和初步鉴定.pdfVIP

贵州农业科学 2011 .39(们: 154-157 Guizhou Agricultural Sciences [文意编每]1001-3601( 201 1) 02-0116-0154-04 养殖水体氨氮降解菌的分离和初步鉴定 易忆，容元平，程谦伟，黎娅，黄飞 〈广西工学院生物与化学工程系.广西柳州 545006) [摘 要]为研究微生物降解和转化水体中的是氮，进行了养殖水体氨氮降解菌的分离和初步鉴定试 验。结采表明，以(NH.)zSO. 为唯一氮源的选择性培养基，通过乎板稀锋分离，从养殖池塘0/筛选出对氨氮 具有高降解活力的菌株 Xl. 当事L 且初始浓度为 50 rng/L 时，永活化的和治化的 Xl 菌在 48h 和 24h 内的战 氨降解率分别为 98.4%和 97.7% 。经形态鉴定以及生理生化试验，初步确定 Xl 菌为巨大芽抱杆菌(βacil伽 lus megaterium )。 [关键调〕成氮降解5 分离接定s 养殖水体;芽抱杆菌 [中圄分~号] S917. 1 [文献标i只码]A Isolation and Identification of Ammonia Nitrogen Degradation Strains from Aquaculture Water YI Yi. RONG Yuan-ping , CHENG Qian-wei. L1 Ya. HUANG Fei (Ðepørtment 01 Biology ønd Chemicøl Engineering. Guangxi Technícøl ωllege. Liu:z.hou. Guøngxi 545006. Chinø) Abstract: Six arnrnonia nitrogen degradation straÎns were Îsolated and identified from aquaculture water to study their degradation and conversion of ammonia nitrogen in aquaculture water. The results showed that Xl strain with a high degradation activity of amrnonía nitrogen was isolated from aquaculture water by plate dilution based on the selective rnediurn with (NH.)2 SO. only. and the arnrnonia nitrogen degradation rate of the non响activated and activated Xl strain was 98. 4 % and 97. 7 % within 48 h and 24 h respectively when the initial concentration of amrnonia nitrogen was 50 mg/L. Xl strain was identified as BaciUus megateríum by rnorphological identification and physiological and biochernical tests. Key words: ammonia nitrogen degradation; ísolation and identificatíon; aquaculture water; bacillus 水产拌殖业是战国植业的重耍组成部分，政直在 步的生理生化鉴炬，为开发多种樊的高敢做生物制 开放以来，找国水产养殖业迅握发展。随着神殖规