8-二维液相色谱分离测定辛夷中的木酚素.pdf

i:/3b2/Jobs/Jss/2007/Heft15/98.3d 2. 7. 2007 J. Sep. Sci. 2007, 30, 0000 – 0000 L. Rieux et al. 1 Weiquan Zhao* Original Paper Tingting Zhou* Guorong Fan Yifeng Chai Isolation and purification of lignans from Magnolia Yutian Wu biondii Pamp by isocratic reversed-phase two- Shanghai Key Laboratory for dimensional liquid chromatography following Pharmaceutical Metabolite microwave-assisted extraction Research, School of Pharmacy, Second Military Medical University, Shanghai, China The dried flower buds of Magnolia biondii Pamp are one of the most widely used medicinal plants officially listed in the Chinese Pharmacopoeia. A 2-D column- switching system without sample loop trapping, where two columns were switched directly via a six-port two-position switching valve, was successfully applied for the first time to the isolation and purification of five lignans including pinoresinol dimethyl ether, magnolin, epi-magnolin A, fargesin, and demethoxyaschantin from M. biondii Pamp after microwave-assisted extraction. The introduction of the six-port switching valve instead of sample loop assured 100% recovery from the first dimen- sion to the second, and the injection volumes of the second dimension could reach 12 mL. In this mode of operation, the so