猪源沙门氏菌携带Ⅰ类整合子向人源细菌体外转移的研究.pdfVIP

又撑农JI:.科学 Ti呗;in Agricultural Sciences 2015 ,21(10):16-18 ·畜牧兽医与水产养殖 猪源沙门氏菌携带I 类整合子向人源细菌体外转移的研究 王学君 1 ，卢春洪 l ，王源 l ，谭艾娟 l ，吕世明 2 ，蒋庆庆 l ，符东)1顶 l ( 1.贵州大学生命科学学院，贵州贵阳 55∞25;2.贵州大学动物科学学院，贵州贵阳 550025 ) 摘 要:为考察猪源沙门氏菌携带的 I 类整合子向人源细菌在体外接合试验中的转移频率，在体外接合试验中应用 PCR 方法 3 检测细菌间 I 类整合子的转移。结果表明:人源沙门氏菌I 类整合子转移效率在1.6x10- -2.5x lO-4 cfu mL咐共体菌)之间，人源 5 1 大肠杆菌I 类整合子转移效率在1.5x lO- -8.3x lO.{; cfu mL- (供体菌)之间，猪源沙门氏菌 I 类整合子未转移给人源金黄色葡萄 球菌。因此，猪源沙门氏菌I 类整合子携带耐药基因盒在体外可向人源革兰氏阴性菌发生转移，且转移频率较高。 关键词:沙门氏菌;体外接合试验;整合子;转移 申图分类号:R446.5 文献标i只码 :A DOI 编码: JO.3969/j.issn.1006 - 6500.2015. 10.004 Research on Class 1 Integron Transmitting to Human Bacteria in Swine Salmonella in Vitro 1 1 1 2 1 WANG Xue-jun , LU Chun-hong , WANG Yuan , TAN Ai-juan I, LYU Shi-ming , JIANG Qing-qing , FU Dong-shun 1 (1. College of Life Science , Guizhou University , Guiyang , Guizhou 55∞25， China; 2. College of Animal Science , Guizhou Universi- ty ,Guiyang , Guizhou 5S0025 ,China) Abstract: To investigate the transfer frequency of class 1 integrons from swine Salmonella to human bacteria on the conjugation test in vitro , the transfertation of class 1 integrons between the strains was deteπnined with PCR method. Results showed that the transferta- 3 tion rate of class 1 integrons from swine Salmonella to human Escherichia coli were in 1.6x 10- -2.5x 10-4 cfu mL -1 (donor strain) , and to human Salmonella were 1.5x lO-5 -8.3x1俨 cfu . mL -1 (donor strain). Class 1 integrons were no found in human in human Staphylococcωαureω. So , class 1 integron